TARGETED DISRUPTION OF THE GATA3 GENE CAUSES SEVERE ABNORMALITIES IN THE NERVOUS-SYSTEM AND IN FETAL LIVER HEMATOPOIESIS
TARGETED DISRUPTION OF THE GATA3 GENE CAUSES SEVERE ABNORMALITIES IN THE NERVOUS-SYSTEM AND IN FETAL LIVER HEMATOPOIESIS
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DOI：
10.1038/ng0995-40
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发表时间：
1995-09-01
期刊：
影响因子：
30.8
通讯作者：
LINDENBAUM, MH
中科院分区：
文献类型：
--
作者：
PANDOLFI, PP;ROTH, ME;LINDENBAUM, MH
GATA-3 是不断增长的相关转录因子家族的成员之一，该家族在所有脊椎动物中都具有高度保守的表达模式。为了使用直接遗传方法阐明 GATA-3 功能，我们通过胚胎干细胞中的同源重组破坏了小鼠基因。 GATA3突变杂合子小鼠具有生育能力，各方面均正常，而纯合突变体胚胎在交配后第11天至12天之间死亡，并表现出大量内出血、明显生长迟缓、大脑和脊髓严重畸形以及胎儿肝脏造血功能严重畸变。
GATA-3 is one member of a growing family of related transcription factors which share a strongly conserved expression pattern in all vertebrate organisms. In order to elucidate GATA-3 function using a direct genetic approach, we have disrupted the murine gene by homologous recombination in embryonic stem cells. Mice heterozygous for the GATA3 mutation are fertile and appear in all respects to be normal, whereas homozygous mutant embryos die between days 11 and 12 postcoitum (p.c.) and display massive internal bleeding, marked growth retardation, severe deformities of the brain and spinal cord, and gross aberrations in fetal liver haematopoiesis.