Cytoplasmic free-Ca2+ level rises with repellents and falls with attractants in Escherichia coli chemotaxis.
Cytoplasmic free-Ca2+ level rises with repellents and falls with attractants in Escherichia coli chemotaxis.
复制标题
在大肠杆菌趋化作用中，细胞质游离 Ca2 水平随驱避剂升高而随引诱剂降低。
DOI：
10.1073/pnas.92.23.10777
复制
发表时间：
1995
影响因子：
11.1
通讯作者：
Adler,J
中科院分区：
文献类型：
--
作者：
Tisa,LS;Adler,J
用荧光Ca（2+）指示剂染料Fura-2测定大肠杆菌胞质游离Ca（2+）水平。化学活性野生型E.大肠杆菌调节细胞质游离Ca 2+在约100 nM时，没有遇到刺激，但细菌行为的变化与细胞质游离Ca 2+浓度的变化相关。对于具有化学活性的野生型E.大肠杆菌，另外的驱避剂导致细胞翻滚和细胞质游离Ca 2+水平的瞬时增加。相反，除了野生型细胞的引诱剂引起的运行，并产生了短暂的细胞质游离Ca 2+水平下降。与突变株的研究表明，所观察到的细胞质游离Ca 2+水平的变化，在响应化学刺激所需的化学感受器。
Cytoplasmic free-Ca2+ levels in Escherichia coli were measured by use of the fluorescent Ca(2+)-indicator dye fura-2. Chemotactically wild-type E. coli regulated cytoplasmic free Ca2+ at approximately 100 nM when no stimuli were encountered, but changes in bacterial behavior correlated with changes in cytoplasmic free-Ca2+ concentration. For chemotactically wild-type E. coli, addition of a repellent resulted in cells tumbling and a transient increase in cytoplasmic free-Ca2+ levels. Conversely, addition of an attractant to wild-type cells caused running and produced a transient decrease in cytoplasmic free-Ca2+ levels. Studies with mutant strains showed that the chemoreceptors were required for the observed changes in cytoplasmic free-Ca2+ levels in response to chemical stimuli.