COMPARISON OF PROSTATIC AND NON-PROSTATIC ACID-PHOSPHATASE
COMPARISON OF PROSTATIC AND NON-PROSTATIC ACID-PHOSPHATASE
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DOI：
10.1111/j.1749-6632.1982.tb40300.x
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发表时间：
1982-01-01
影响因子：
5.2
通讯作者：
HACKER, B
中科院分区：
文献类型：
--
作者：
LAM, KW;LI, CY;HACKER, B
应用电泳和离子交换柱层析分离了多种具有不同生物学和临床意义的酸性磷酸酶。带0在具有许多自噬空泡的腹水细胞中非常强，表明在自噬功能中的作用。带1是膜结合的酸性磷酸酶，主要见于微粒体组分。带3是所有非前列腺组织的主要溶酶体酸性磷酸酶。条带2和4在抗原性上彼此相同，并且在前列腺中观察到异常高的量。条带2和4之间的不同电泳迁移率是由于它们的碳水化合物含量。带5是破骨细胞的特征性酶。酒石酸盐敏感酶包括条带0至4。只有第5带是抗酒石酸盐的。电泳法未检测到红细胞抗酒石酸酸性磷酸酶。对于带2和带5均观察到临床应用。带2是一种分泌酶，通常分泌到精浆中。在某些前列腺癌患者中，带2被吸收到血液循环中。在非前列腺组织中观察到少量条带2和4。条带2的诊断价值是由于其在前列腺中的极高浓度。带5在正常前列腺中未观察到。在毛细胞、戈谢细胞和破骨细胞中观察到高浓度的条带5。血清带5b水平是骨中骨细胞活性的指标。在正常儿童生理骨生长期间、戈谢病和恶性肿瘤骨转移中观察到血清带5b升高。
Electrophoresis and ion-exchange column chromatography were used to separate the wide varieties of acid phosphatases with different biological and clinical significance. Band 0 was very strong in ascitic cells with many autophagic vacuoles, indicating a role in autophagic function. Band 1 was a membrane-bound acid phosphatase, seen mainly in the microsomal fraction. Band 3 was the major lysosomal acid phosphatase of all nonprostatic tissues. Bands 2 and 4 were antigenically identical to each other, and were observed in unusually high amounts in the prostate. The different electrophoretic mobility between bands 2 and 4 was due to their carbohydrate content. Band 5 was a characteristic enzyme of the osteoclast. The tartrate-sensitive enzymes included bands 0 through 4. Only band 5 was tartrate resistant. The tartrate-resistant acid phosphatase of erythrocytes was not detected by the electrophoresis method. Clinical applications were seen for both bands 2 and 5. Band 2 was a secretory enzyme, normally secreted into the seminal plasma. Band 2 was absorbed into the blood circulation in some prostatic cancer patients. A small amount of bands 2 and 4 was observed in nonprostatic tissues. The diagnostic value of band 2 resulted from its extremely high concentration in the prostate. Band 5 was not observed in the normal prostate. A high concentration of band 5 was observed in hairy cells, Gaucher cells, and osteoclasts. The serum level of band 5b was an indicator of osteoclastic activity in the bone. Elevation of band 5b in serum was observed in normal children during physiological bone growth, in Gaucher's disease, and in malignancies metastasized to bone.