Comparative RNA-seq analysis of transcriptome dynamics during petal development in Rosa chinensis.
Comparative RNA-seq analysis of transcriptome dynamics during petal development in Rosa chinensis.
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DOI：
10.1038/srep43382
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发表时间：
2017-02-22
影响因子：
4.6
通讯作者：
Zhang Q
中科院分区：
文献类型：
--
作者：
Han Y;Wan H;Cheng T;Wang J;Yang W;Pan H;Zhang Q
中国玫瑰（Rosa chinensis）华丽花瓣的发育过程是复杂的，被认为依赖于一系列功能多样的基因的平衡表达；然而，玫瑰花瓣发育的分子基础在很大程度上是未知的。本研究将红薯（R. chinensis）品种‘Old Blush’的花瓣生长分为4个发育阶段，并利用RNA-seq技术分析了随着发育进程而发生的转录动态变化。总共获得了5.98亿个clean reads和61456个成功注释的unigenes。差异表达基因（DEG）分析比较了发育阶段的转录组，从而确定了几个潜在的参与花瓣发育的候选基因。除了几个候选转录因子外，还深入研究了参与花青素生物合成、花瓣扩张和植物激素途径的deg。这些结果为进一步研究玫瑰花瓣发育的调控机制奠定了基础，并可用于培育具有理想性状的观赏玫瑰分子育种计划。
The developmental process that produces the ornate petals of the China rose (Rosa chinensis) is complex and is thought to depend on the balanced expression of a functionally diverse array of genes; however, the molecular basis of rose petal development is largely unknown. Here, petal growth of the R. chinensis cultivar ‘Old Blush’ was divided into four developmental stages, and RNA-seq technology was used to analyse the dynamic changes in transcription that occur as development progresses. In total, 598 million clean reads and 61,456 successfully annotated unigenes were obtained. Differentially expressed gene (DEG) analysis comparing the transcriptomes of the developmental stages resulted in the identification of several potential candidate genes involved in petal development. DEGs involved in anthocyanin biosynthesis, petal expansion, and phytohormone pathways were considered in depth, in addition to several candidate transcription factors. These results lay a foundation for future studies on the regulatory mechanisms underlying rose petal development and may be used in molecular breeding programs aimed at generating ornamental rose lines with desirable traits.