N-HYDROXYSULFOSUCCINIMIDE ACTIVE ESTERS - BIS(N-HYDROXYSULFOSUCCINIMIDE) ESTERS OF 2 DICARBOXYLIC-ACIDS ARE HYDROPHILIC, MEMBRANE-IMPERMEANT, PROTEIN CROSS-LINKERS
N-HYDROXYSULFOSUCCINIMIDE ACTIVE ESTERS - BIS(N-HYDROXYSULFOSUCCINIMIDE) ESTERS OF 2 DICARBOXYLIC-ACIDS ARE HYDROPHILIC, MEMBRANE-IMPERMEANT, PROTEIN CROSS-LINKERS
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DOI：
10.1021/bi00260a008
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发表时间：
1982-01-01
期刊：
影响因子：
2.9
通讯作者：
STAROS, JV
中科院分区：
文献类型：
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作者：
STAROS, JV
合成了一种用于制备活性酯的新型亲水性配体n -羟基磺基琥珀酰亚胺，并对其进行了表征。该配体被纳入2种新的蛋白质交联试剂3,3′-二硫代丙酸磺基琥珀酰亚基和双硫代琥珀酰亚基亚酸。在兔肌醛缩酶的实验中，这两种试剂在生理pH下都是高效的蛋白质交联剂，在温和的条件下，3,3”-二硫代比斯（磺基琥珀酰丙酸）可以通过还原进行定量切割。在完整的人红细胞和红细胞膜的实验中，这两种试剂都是不含膜的，当红细胞用任何一种试剂处理时，阴离子通道（带3）的亚基都与胞质外膜表面的共价二聚体交联。
N-hydroxysulfosuccinimide, a new hydrophilic ligand for the preparation of active esters was synthesized and characterized. This ligand was incorporated into 2 new protein cross-linking reagents, 3,3''-dithiobis(sulfosuccinimidyl propionate) and bis(sulfosuccinimidyl) suberate. In experiments with rabbit muscle aldolase, both of these reagents are highly efficient protein cross-linkers at physiological pH and 3,3''-dithiobis(sulfosuccinimidyl propionate) is quantitatively cleavable by reduction under mild conditions. In experiments with intact human erythrocytes and erythrocyte membranes, both reagents are membrane impermeant and when erythrocytes are treated with either reagent, both cross-link subunits of the anion channel (band 3) to covalent dimers at the extracytoplasmic membrane face.