N-3 Polyunsaturated Fatty Acids Improve Liver Lipid Oxidation-Related Enzyme Levels and Increased the Peroxisome Proliferator-Activated Receptor α Expression Level in Mice Subjected to Hemorrhagic Shock/Resuscitation.
N-3 Polyunsaturated Fatty Acids Improve Liver Lipid Oxidation-Related Enzyme Levels and Increased the Peroxisome Proliferator-Activated Receptor α Expression Level in Mice Subjected to Hemorrhagic Shock/Resuscitation.
复制标题
N-3 多不饱和脂肪酸可改善失血性休克/复苏小鼠的肝脏脂质氧化相关酶水平并增加过氧化物酶体增殖物激活受体 α 表达水平
作者：
Zhang L;Tian F;Gao X;Wang X;Wu C;Li N;Li J
失血性休克/复苏损伤后适当的代谢干预尚未确定。我们的目的是研究鱼油对失血性休克/复苏后脂质代谢干预的影响。首先，将48只C57 BL/6小鼠分配到六组（每组n = 8）。假手术组不进行手术，而其余组中的小鼠在失血性休克/复苏后1-5天处死。在第二部分中，在损伤后5天用盐水或鱼油处理小鼠（每组n = 8）。我们测定了血清甘油三酯水平，收集并制备肝组织用于qRT-PCR或Western印迹分析。我们发现失血性休克/复苏后5天甘油三酯水平升高，但添加鱼油后降低。损伤后，肝组织中肉毒碱棕榈酰转移酶1A、脂肪酸转运蛋白1和过氧化物酶体增殖物激活受体α的蛋白和基因表达均明显下降。相反，鱼油处理后，这些靶点的表达水平与生理盐水组相比有所增加。提示n-3多不饱和脂肪酸可改善失血性休克/复苏后肝脏脂质氧化相关酶活性。这一功能可能是通过激活过氧化物酶体增殖物激活受体-α途径实现的。
Appropriate metabolic interventions after hemorrhagic shock/resuscitation injury have not yet been identified. We aimed to examine the effects of fish oil on lipid metabolic intervention after hemorrhagic shock/resuscitation. Firstly, 48 C57BL/6 mice were assigned to six groups (n = 8 per group). The sham group did not undergo surgery, while mice in the remaining groups were sacrificed 1–5 days after hemorrhagic shock/resuscitation. In the second part, mice were treated with saline or fish oil (n = 8 per group) five days after injury. We determined serum triglyceride levels and liver tissues were collected and prepared for qRT-PCR or Western blot analysis. We found that triglyceride levels were increased five days after hemorrhagic shock/resuscitation, but decreased after addition of fish oil. After injury, the protein and gene expression of carnitine palmitoyltransferase 1A, fatty acid transport protein 1, and peroxisome proliferator-activated receptor-α decreased significantly in liver tissue. In contrast, after treatment with fish oil, the expression levels of these targets increased compared with those in the saline group. The present results suggest n-3 polyunsaturated fatty acids could improve lipid oxidation-related enzymes in liver subjected to hemorrhagic shock/resuscitation. This function is possibly accomplished through activating the peroxisome proliferator-activated receptor-α pathway.