Individual variability in the stress response of C57BL/6J male mice correlates with trait anxiety
Individual variability in the stress response of C57BL/6J male mice correlates with trait anxiety
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DOI：
10.1111/j.1601-183x.2007.00345.x
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发表时间：
2008-03-01
影响因子：
2.5
通讯作者：
Morellini, F.
中科院分区：
文献类型：
--
作者：
Jakovcevski, M.;Schachner, M.;Morellini, F.
压力强烈地改变了一些人的生理和行为，而另一些人则很少或没有受到影响。这种个体差异的原因仍然未知。在这里，我们假设，表观遗传诱导的特质焦虑水平预测个体小鼠在遗传同质群体的应激反应。选择近交系C57 BL/6雄性小鼠的潜伏期，以自由地从其家笼进入不熟悉的竞技场，并将其分类为具有高或低水平的特质焦虑。然后将小鼠暴露于急性应激（与大鼠1小时的嗅觉接触）或对照条件。24小时后，急性应激增强了状态焦虑，仅在先前被归类为具有高水平特质焦虑的小鼠中进行了高架十字迷宫测试。这种急性应激的焦虑作用是通过增强新奇诱导的血浆皮质酮分泌和增加海马中糖皮质激素和盐皮质激素受体的信使RNA（mRNA）表达来消除的。在被归类为具有低水平特质焦虑的小鼠中没有观察到急性应激的影响。在非应激对照条件下，小鼠仅在海马糖皮质激素受体mRNA的基础水平上存在差异，高特质焦虑小鼠的水平高于低特质焦虑小鼠。总之，近交系C57 BL/6小鼠显示出显着高的个体间变异性，在他们的特质焦虑，预测行为和神经内分泌反应的急性应激源，表明表达的极端不同的应对策略也可以发展之间的遗传相同的个人。
Stress strongly alters the physiology and behavior of some individuals, while others are little or not affected. The causes of this individual variability have remained unknown. Here, we hypothesize that epigenetically induced levels of trait anxiety predict the stress response of individual mice in a genetically homogeneous population. Inbred C57BL/6 male mice were selected for their latency to freely enter from their home cage into an unfamiliar arena and classified as having high or low levels of trait anxiety. Mice were then exposed to acute stress (1-h olfactory contact with a rat) or control conditions. After 24 h, acute stress enhanced state anxiety measured in the elevated-plus maze test only in mice previously classified as having high levels of trait anxiety. This anxiogenic effect of acute stress was paralleled by enhanced novelty-induced plasma corticosterone secretion and increased messenger RNA (mRNA) expression for glucocorticoid and mineralocorticoid receptors in the hippocampus. No effects of acute stress were observed in mice classified as having low levels of trait anxiety. Under unstressed control conditions, mice only differed in basal levels of hippocampal mRNA for the glucocorticoid receptor, which were higher in mice with high trait anxiety than in mice with low trait anxiety. In summary, inbred C57BL/6 mice display a remarkably high interindividual variability in their trait anxiety that predicts the behavioral and neuroendocrine response to an acute stressor, indicating that expression of extremely different coping strategies can develop also between genetically identical individuals.