Interleukin 5 Plays an Essential Role in Elicitation of Contact Sensitivity through Dual Effects on Eosinophils and B-1 Cells
Interleukin 5 Plays an Essential Role in Elicitation of Contact Sensitivity through Dual Effects on Eosinophils and B-1 Cells
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白细胞介素 5 通过对嗜酸性粒细胞和 B-1 细胞的双重作用，在引发接触敏感性方面发挥重要作用
DOI：
10.1159/000092705
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发表时间：
2006
影响因子：
2.8
通讯作者：
K. Takatsu
中科院分区：
文献类型：
--
作者：
A. Itakura;Y. Kikuchi;T. Kouro;M. Ikutani;S. Takaki;P. Askenase;K. Takatsu
背景资料：接触敏感性（CS）的诱发依赖于B-1细胞衍生的抗原特异性免疫球蛋白M（IgM）抗体，该抗体将CS效应T细胞募集到局部组织中，随后是抗原非特异性单核细胞和多形核细胞（如中性粒细胞和嗜酸性粒细胞）的浸润。在这项研究中，我们研究了白细胞介素（IL）-5，这对嗜酸性粒细胞和B-1细胞有广泛的影响，在CS的诱发作用。方法：采用恶唑酮半抗原对IL-5受体α链缺陷（IL-5 R α-/-）小鼠和IL-5 R α+/+小鼠进行接触致敏。4天后，用相同的半抗原攻击小鼠，并在攻击后24小时测量耳肿胀反应。通过皮肤组织学和嗜酸性粒细胞过氧化物酶活性检查确定嗜酸性粒细胞浸润到局部组织中。为了研究IL-5在B-1细胞活化中的作用，通过酶联免疫斑点试验测定脾脏中恶唑酮特异性IgM产生细胞的数量。结果如下：IL-5 R α-/-小鼠的耳肿胀反应约为IL-5 R α+/+小鼠的一半，在IL-5 R α-/-小鼠皮肤中几乎未观察到嗜酸性粒细胞浸润。致敏和激发的IL-5 R α-/-小鼠的嗜酸性粒细胞过氧化物酶活性比免疫的IL-5 R α+/+小鼠低约11倍。在IL-5 R α+/+小鼠脾脏中，接触致敏显著增加了恶唑啉特异性IgM产生细胞的数量，但在IL-5 R α-/-小鼠脾脏中未增加。结论：IL-5依赖性激活嗜酸性粒细胞和B-1细胞是诱导和诱发CS的必要条件。这些发现为CS诱导的复杂机制提供了新的见解，并表明IL-5在免疫应答调节中的新作用。
Background: Elicitation of contact sensitivity (CS) depends on B-1-cell-derived antigen-specific immunoglobulin M (IgM) antibodies that recruit CS effector T cells into the local tissue, which is followed by infiltration of antigen-nonspecific mononuclear cells and polymorphonuclear cells, such as neutrophils and eosinophils. In this study, we investigated the role of interleukin (IL)-5, which has broad effects on both eosinophils and B-1 cells, in elicitation of CS. Methods: IL-5 receptor α-chain-deficient (IL-5Rα–/–) mice and IL-5Rα+/+ mice were contact sensitized with oxazolone hapten. Four days later, mice were challenged with the same hapten, and ear swelling responses were measured at 24 h after challenge. Eosinophil infiltration into the local tissue was determined by examination of skin histology and eosinophil peroxidase activity. To investigate the role of IL-5 in B-1 cell activation, the number of oxazolone-specific IgM-producing cells in the spleen was determined by enzyme-linked immunospot assay. Results: Ear swelling responses in IL-5Rα–/– mice were about half of those in IL-5Rα+/+ mice, and nearly no eosinophil infiltration was observed in IL-5Rα–/– mouse skin. Eosinophil peroxidase activity in the sensitized and challenged IL-5Rα–/– mice was about 11 times less than that in immunized IL-5Rα+/+ mice. Contact sensitization significantly increased in numbers of oxazolne-specific IgM-producing cells in IL-5Rα+/+ mouse spleen, but not in IL-5Rα–/– mouse spleen. Conclusion: We conclude that IL-5-dependent activation of eosinophils and B-1 cells is necessary for induction and elicitation of CS. These findings provide a new insight into complicated mechanisms of CS elicitation and suggest a novel role of IL-5 in the regulation of immune responses.
DOI：
10.1172/jci20479
发表时间：
2004-08
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
C. Binder;K. Hartvigsen;Mi-Kyung Chang;Marina Miller;D. Broide;W. Palinski;L. Curtiss;M. Corr;J. Witztum
通讯作者：
C. Binder;K. Hartvigsen;Mi-Kyung Chang;Marina Miller;D. Broide;W. Palinski;L. Curtiss;M. Corr;J. Witztum
迟发型超敏反应是由两种不同的 T 细胞活性序列介导的。
DOI：
--
发表时间：
1984
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
VanLoveren,H;Askenase,PW
通讯作者：
Askenase,PW