Lasing in Live Mitotic and Non-Phagocytic Cells by Efficient Delivery of Microresonators.
Lasing in Live Mitotic and Non-Phagocytic Cells by Efficient Delivery of Microresonators.
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通过有效传递微谐振器在活有丝分裂细胞和非吞噬细胞中发射激光。
DOI：
10.1038/srep40877
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发表时间：
2017-01-19
影响因子：
4.6
通讯作者：
Gather MC
中科院分区：
文献类型：
--
作者：
Schubert M;Volckaert K;Karl M;Morton A;Liehm P;Miles GB;Powis SJ;Gather MC
迫切需要可靠的方法来真实的实时单独跟踪大量细胞，以促进我们对癌症转移，神经元网络发育和伤口愈合等重要生物过程的理解。最近有人建议将显微镜回音壁模式激光器引入细胞的细胞质中，并使用其特征性的、依赖于尺寸的发射光谱作为光学条形码，但到目前为止还没有证据表明这种方法是普遍适用的。在这里，我们描述了一种方法，该方法大大提高了几种细胞类型，包括有丝分裂和非吞噬细胞的共振器的细胞内递送。此外，我们的谐振腔的尺寸对发射的激光的光谱特性的影响，并确定一个最佳的尺寸范围，便于标记和跟踪的数千个细胞同时。最后，我们观察到微谐振器在细胞分裂过程中仍然被细胞内化，这使得能够标记几代细胞。
Reliable methods to individually track large numbers of cells in real time are urgently needed to advance our understanding of important biological processes like cancer metastasis, neuronal network development and wound healing. It has recently been suggested to introduce microscopic whispering gallery mode lasers into the cytoplasm of cells and to use their characteristic, size-dependent emission spectrum as optical barcode but so far there is no evidence that this approach is generally applicable. Here, we describe a method that drastically improves intracellular delivery of resonators for several cell types, including mitotic and non-phagocytic cells. In addition, we characterize the influence of resonator size on the spectral characteristics of the emitted laser light and identify an optimum size range that facilitates tagging and tracking of thousands of cells simultaneously. Finally, we observe that the microresonators remain internalized by cells during cell division, which enables tagging several generations of cells.