The Preparation of MR Specific USPIO Probes Using One-Pot Method
The Preparation of MR Specific USPIO Probes Using One-Pot Method
复制标题
一锅法制备 MR 特异性 USPIO 探针
DOI：
10.1166/asl.2012.3687
复制
发表时间：
2012-11
影响因子：
8
通讯作者：
Zhang, Zongming
中科院分区：
文献类型：
--
作者：
Chen, Zhengguang;Li, Fei;Wang, Jichen;Zhang, Zongming
专用磁共振探头是实现靶点磁共振成像的关键。靶向探针的尺寸、循环半衰期、磁性以及与生物标记物的结合是设计靶向探针的先决条件。此外，制备过程尽可能简单，便于大规模生产。在此，我们报告使用one-pot method.Material和MethodThe MR分子探针合成羧化葡聚糖包裹的纳米粒子与抗AFP或抗GPC 3抗体通过EDC/NHS介导的反应，合成探针。用透射电镜和动态光散射法测定了探针的物理和化学性质，并在1.5T临床MR扫描仪上与未结合的USPION进行了弛豫性能的比较。通过紫外-可见分光光度计测量抗体与纳米颗粒的结合效率。结果该探针的核心平均直径为5-8 nm，平均流体动力学直径为47 nm，分布较宽，且呈微区分布。抗AFP抗体和抗GPC 3抗体的偶联率分别为88.8%和15.9%。USPIO、抗AFP-USPIO和抗GPC 3-USPIO对T1和T2值的缩短无统计学意义。结论采用一锅法合成MR分子探针简便易行。所合成的探针可作为一种高特异性的体外实验研究试剂，并具有潜在的临床应用价值
The Specific MR Probes are the key to carry out the target MR imaging. The size, half-life in the circulation, magnetism and combination with the biological marker specifically are the prerequisite factors in the design of targetable probes. In addition, the preparation process is as simple as possible for facilitating mass production. Herein, we report MR molecular probes synthesized using one-pot method.Material and MethodThe carboxylated dextran-coated nanoparticles were conjugated with anti-AFP or anti-GPC3 antibodies through EDC/NHS-mediated reaction to synthesize the probes. The physical and chemical properties of the probes were determined by TEM and DLS, and the relaxivity was compared to uncombined USPIONs using 1.5T clinical MR scanner. The binding efficiency of the antibodies to nanoparticles was measured by an ultraviolet-visible spectrophotometer. In addition, the probes were incubated with targetable cells in vitro.ResultThe results showed that the core mean diameter of the probe was 5–8 nm and average hydrodynamic diameter was 47 nm with a broad slightly distribution. The coupling efficiency of anti-AFP and anti-GPC3 antibodies was 88.8% and 15.9% respectively. There was no statistically significant about shortening the T1 and T2 values of agar solution among USPIO, anti-AFP-USPIO and antiGPC3-USPIO. These two probes can enter the targetable cells, respectively.ConclusionIt is simple and convenient to synthesize the MR molecular probes using one-pot method. The synthesized probes can be used as a high specific agent for experimental study in vitro and to have potential clinical MR imaging