TANG01 recruits ERG IC membranes to the endoplasmic reticulum for procollagen export
TANG01 recruits ERG IC membranes to the endoplasmic reticulum for procollagen export
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DOI：
10.7554/elife.10982
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发表时间：
2015-11-14
期刊：
影响因子：
7.7
通讯作者：
Malhotra, Vivek
中科院分区：
文献类型：
--
作者：
Santos, Antonio J. M.;Raote, Ishier;Malhotra, Vivek
之前我们发现，依靠tango - 1从内质网（ER）输出VII前胶原需要膜融合（Nogueira等）。, 2014)。与t-SNARE Syntaxin 18一起，我们现在揭示了这一过程中所需的SNAREs的完整补充，t-SNAREs BNIP1和USE1，以及v -SNARE YKT6。TANGO1招募含有ykt6的内质网高尔基中间隔室（ERGIC）膜到内质网富含前胶原的斑块。此外，残基1214-1396，包括TANGO1的第一个卷曲线圈，即使在靶向线粒体时也能特异性地招募ERGIC膜。因此，TANGO1在集中管腔内的前胶原VII和在内质表面募集ERGIC膜方面起着关键作用。我们的数据显示，从内质网输出胶原的巨型运输载体的生长不是通过获取更大的内质网膜斑块，而是通过tango - 1介导的过程将ERGIC膜添加到内质网富含前胶原的结构域。
Previously we showed that membrane fusion is required for TANGO1-dependent export of procollagen VII from the endoplasmic reticulum (ER) (Nogueira, et a1., 2014). Along with the t -SNARE Syntaxin 18, we now reveal the complete complement of SNAREs required in this process, t-SNAREs BNIP1 and USE1, and v -SNARE YKT6. TANGO1 recruits YKT6-containing ER Golgi Intermediate Compartment (ERGIC) membranes to procollagen VII -enriched patches on the ER. Moreover residues 1214-1396, that include the first coiled coil of TANGO1, specifically recruit ERGIC membranes even when targeted to mitochondria. TANGO1 is thus pivotal in concentrating procollagen VII in the lumen and recruiting ERGIC membranes on the cytoplasmic surface of the ER. Our data reveal that growth of a mega transport carrier for collagen export from the ER is not by acquisition of a larger patch of ER membrane, but instead by addition of ERGIC membranes to procollagen-enriched domains of the ER by a TANGO1-mediated process.