Molecular study of microsporidiosis due to Enterocytozoon bieneusi and Encephalitozoon intestinalis among human immunodeficiency virus-infected patients from two geographical areas:: Niamey, Niger, and Hanoi, Vietnam
Molecular study of microsporidiosis due to Enterocytozoon bieneusi and Encephalitozoon intestinalis among human immunodeficiency virus-infected patients from two geographical areas:: Niamey, Niger, and Hanoi, Vietnam
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DOI：
10.1128/jcm.00684-07
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发表时间：
2007-09-01
影响因子：
9.4
通讯作者：
Gay-Andrieu, Francoise
中科院分区：
文献类型：
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作者：
Espern, Anne;Morio, Florent;Gay-Andrieu, Francoise
由bieneusenterocytozoon和Encephalitozoon ininalis引起的微孢子虫病病例是与人类广泛临床综合征相关的新出现的机会性感染。本研究的目的是明确两个不同地理区域的微孢子流行病学。2004年11月至2005年8月，分别在尼日尔尼亚美和越南河内采集了228份和42份粪便样本。采用紫外显微镜对微孢子虫进行筛选。采用两种特异的分子生物学检测方法，证实了该方法的有效性。所有阳性标本均进行基因分型。在这项研究中，我们发现人类免疫缺陷病毒感染患者的微孢子虫病患病率很高，在尼亚美和河内分别为10.5%和9.5%。这些流行水平与欧洲国家在采用高活性抗逆转录病毒疗法之前的记录相似。在阳性的样本中，我们发现了七种不同的基因型，包括两种以前未描述的基因型。两个地理区域的布氏伊氏体基因型分布表明存在不同的感染传播途径，尼日尔是人传人，越南是人畜共患。
Microsporidiosis cases due to Enterocytozoon bieneusi and Encephalitozoon intestinalis are emerging opportunistic infections associated with a wide range of clinical syndromes in humans. The aim of this study was to specify microsporidial epidemiology in two different geographical areas. From November 2004 to August 2005, 228 and 42 stool samples were collected in Niamey, Niger, and Hanoi, Vietnam, respectively. Screening for microsporidia was performed using UV-Iight microscopy. Detection was confirmed by molecular biology using two methods specific for E. bieneusi and E. intestinalis. All samples positive for E. bieneusi were subjected to genotyping. In this study, we found high prevalences of microsporidiosis among human immunodeficiency virus-infected patients, 10.5% and 9.5%, respectively, in Niamey and Hanoi. These levels of prevalence are similar to those recorded in European countries before highly active antiretroviral therapy was introduced. In the samples positive for E. bieneusi, we found seven distinct genotypes, including two genotypes not previously described. The E. bieneusi genotype distributions in the two geographical areas suggest different routes of infection transmission, person-to-person in Niger and zoonotic in Vietnam.