Plant development from microspore-derived embryos in oilseed rape as affected by chilling, desiccation and cotyledon excision
Plant development from microspore-derived embryos in oilseed rape as affected by chilling, desiccation and cotyledon excision
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DOI：
10.1007/s10535-006-0004-6
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发表时间：
2006-06
影响因子：
1.5
通讯作者：
G. Zhang;D. Zhang;G. Tang;Y. He;W. Zhou
中科院分区：
文献类型：
--
作者：
G. Zhang;D. Zhang;G. Tang;Y. He;W. Zhou
以油菜为材料，研究了低温、部分脱水、子叶切除和小孢子胚继代培养对油菜植株发育的影响。结果表明，在五种培养基中，当胚胎培养在含有2.0 mg dm− 3苄氨基嘌呤的半强度Murashige和Skoog培养基上时，所有基因型均表现出最佳反应。低温处理3 ~ 5d可进一步提高胚的萌发率（90.0%）和成苗率（58.46%）。干燥一天也增加了所有基因型的胚萌发和植株发育。在子叶期后期切取胚的子叶，可显著提高成苗率。以小于4.0 mm的胚为外植体，继代培养进一步提高了胚的萌发率和成苗率。基因型ZJU 452胚状体第二次继代培养成苗率达99.78%。
The present study evaluated the effects of chilling, partial desiccation, cotyledon excision and successive subculture of microspore-derived embryos on plant development in oilseed rape (Brassica napusL.). The results showed that out of the five media, all the genotypes showed the best response when the embryos were cultured on the half-strength Murashige and Skoog medium with 2.0 mg dm−3benzylaminopurine. A cold treatment for 3 or 5 d further increased frequencies of embryo germination (90.0 %) and plantlet development (58.46 %). Desiccation for one day also increased the embryo germination and plantlet development in all genotypes tested. Cutting the cotyledons of the embryos at late cotyledonary stage significantly increased the frequency of plantlet development. The highest rate of plantlet development was obtained from cultures of embryos sampled with size of less than 4.0 mm. The successive subculture further improved the germination and development of plantlets from embryos. In the genotype ZJU452, the rate of plantlet development reached 99.78 % after the second subculture of embryos.