Forskolin promotes astroglial differentiation of human central neurocytoma cells
Forskolin promotes astroglial differentiation of human central neurocytoma cells
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DOI：
10.1038/emm.2004.7
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发表时间：
2004-02-29
影响因子：
12.8
通讯作者：
Suh-Kim, HY
中科院分区：
文献类型：
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作者：
Kim, BJ;Kim, SS;Suh-Kim, HY
人中枢神经细胞瘤是一种常见于侧脑室前部的脑肿瘤。在本研究中，我们建立了神经细胞瘤细胞培养增殖的条件，并在体外分析了神经细胞瘤细胞的特征。体外培养神经干细胞时，含有碱性成纤维细胞生长因子（bFGF）的条件具有较高的增殖能力。RT-PCR分析显示，神经细胞瘤细胞中存在巢蛋白，提示神经细胞瘤具有神经干细胞的特性。有趣的是，用forskolin治疗神经细胞瘤细胞增加了胶质纤维酸性蛋白的表达，同时降低了巢蛋白的表达。Forskolin还诱导神经细胞瘤细胞形态改变，呈星形细胞样表型。提示神经细胞瘤细胞可能具有多能性神经干细胞的特性。
Human central neurocytoma is a kind of the brain tumors that are usually found in anterior part of the lateral ventricles. In this study, we established conditions that allowed proliferation of neurocytoma cells culture and analyzed characteristics of neurocytoma cells in vitro. For in vitro, a condition that used for culturing neural stem cells and contained basic fibroblast growth factor (bFGF) provided high proliferation. RT-PCR analaysis showed that nestin was found in neurocytoma cells, indicating that the neurocytomas possess neural stem cell properties. Interestingly, treatment of neurocytoma cells with forskolin increased expression of glial fibrillary acidic protein with a concomitant decrease in the nestin expression. Forskolin also induced morphological changes of neurocytoma cells to adopt an astrocyte-like phenotype. The results suggest that neurocyotma cells may have properties of multipotent neural stem cells.