Anti-inflammatory role of CD11b+Ly6G+ neutrophilic cells in allergic airway inflammation in mice
Anti-inflammatory role of CD11b+Ly6G+ neutrophilic cells in allergic airway inflammation in mice
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DOI：
10.1016/j.imlet.2018.10.007
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发表时间：
2018-12-01
影响因子：
4.4
通讯作者：
Kolahian, Saeed
中科院分区：
文献类型：
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作者：
Nowroozilarki, Negar;Oez, Hasan Halit;Kolahian, Saeed
哮喘是一种慢性炎症性疾病，由辅助性T细胞2型(Th2)反应过度激活引起。在本研究中，我们研究了CD11b(+)Ly6G(+)中性粒细胞在体内过敏性气道炎症中的功能相关性。用屋尘螨(HDM)或卵白蛋白(OVA)致敏和激发诱导小鼠过敏性呼吸道炎症。流式细胞仪检测CD11b(+)、Ly6G(+)中性粒细胞和T细胞表型。为了评估体内功能的相关性，将CD11b(+)Ly6G(+)中性粒细胞过继转移到静脉或气管内，并研究其对呼吸道炎症的影响。过继转移的CD11b(+)Ly6G(+)中性粒细胞可在体内减弱Th2和Th17反应和呼吸道炎症。总之，我们的结果表明，CD11b(+)Ly6G(+)中性粒细胞在体内抑制过敏性小鼠的呼吸道炎症。过继细胞转移抑制中性粒细胞可能是一种有吸引力的过敏性呼吸道炎症的治疗策略。
Asthma is a chronic inflammatory disease driven by overactivation of T helper cell type 2 (Th2) responses. In the present study, we investigated the functional relevance of CD11b(+)Ly6G(+) neutrophilic cells in allergic airway inflammation in vivo. Allergic airway inflammation in mice was induced by house dust mite (HDM) or ovalbumin (OVA) sensitization and challenge. CD11b(+)Ly6G(+) neutrophilic cells and T cell phenotypes were quantified by flow cytometry. To assess the functional in vivo relevance, CD11b(+)Ly6G(+) neutrophilic cells were adoptively transferred intravenously or intratracheally and consequences on airway inflammation were studied. Adoptively transferred CD11b(+)Ly6G(+) neutrophilic cells attenuated Th2 and Th17 responses and airway inflammation in vivo. Collectively, our results demonstrate that CD11b(+)Ly6G(+) neutrophilic cells suppress airway inflammation in allergic mice in vivo. Adoptive cellular transfer of suppressive neutrophilic cells may represent an attractive therapeutic strategy for allergic airway inflammation.