Fluorescence-enhanced gadolinium-doped zinc oxide quantum dots for magnetic resonance and fluorescence imaging
Fluorescence-enhanced gadolinium-doped zinc oxide quantum dots for magnetic resonance and fluorescence imaging
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用于磁共振和荧光成像的荧光增强掺钆氧化锌量子点
DOI：
10.1016/j.biomaterials.2010.10.022
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发表时间：
2011-02-01
期刊：
影响因子：
14
通讯作者：
Lu, Lehui
中科院分区：
文献类型：
--
作者：
Liu, Yanlan;Ai, Kelong;Lu, Lehui
我们在这里报告的发展，钆掺杂氧化锌量子点（量子点）作为双模荧光和磁共振成像纳米探针。该方法简单、通用、环境友好，不仅降低了制备的难度和复杂性，而且避免了以往报道的纳米探针合成中二氧化硅包覆过程所带来的粒径增大。这些纳米探针，具有非常小的尺寸和增强的荧光所产生的Gd掺杂，可以成功地标记HeLa细胞在短时间内，并提出没有证据的毒性或对细胞生长的不利影响，即使在浓度高达1毫米。这些结果表明，这样的纳米探针具有低毒性，特别是在与传统的聚乙二醇化的CdSe/ZnS或CdSe/CdS量子点相比。在MRI研究中，它们具有很强的正对比效应，水质子的纵向弛豫率（r（1））为16 mm（-1）s（-1）。它们对HeLa细胞进行MRI成像的能力意味着它们具有作为MRI造影剂的巨大潜力。结合荧光成像的高灵敏度和MRI的高空间分辨率，我们期望所制备的Gd掺杂ZnO量子点能够提供更好的数据可靠性，并在生物、医学等领域有着广阔的应用前景。(C)2010爱思唯尔有限公司版权所有。
We report here the development of Gd-doped ZnO quantum dots (QDs) as dual modal fluorescence and magnetic resonance imaging nanoprobes. They are fabricated in a simple, versatile and environmentally friendly method, not only decreasing the difficulty and complexity, but also avoiding the increase of particle's size brought about by silica coating procedure in the synthesis of nanoprobes reported previously. These nanoprobes, with exceptionally small size and enhanced fluorescence resulting from the Gd doping, can label successfully the HeLa cells in short time and present no evidence of toxicity or adverse affect on cell growth even at the concentration up to 1 mm. These results show that such nanoprobes have low toxicity, especially in comparison with the traditional PEGylated CdSe/ZnS or CdSe/CdS QDs. In MRI studies, they exert strong positive contrast effect with a large longitudinal relaxivity (r(1)) of water proton of 16 mm(-1) s(-1). Their capability of imaging HeLa cells with MRI implies that they have great potential as MRI contrast agents. Combining the high sensitivity of fluorescence imaging with high spatial resolution of MRI, We expect that the as-prepared Gd-doped ZnO QDs can provide a better reliability of the collected data and find promising applications in biological, medical and other fields. (C) 2010 Elsevier Ltd. All rights reserved.