The Vibrio cholerae O139O-antigen polysaccharide is essential for Ca2+-dependent biofilm development in sea water
The Vibrio cholerae O139O-antigen polysaccharide is essential for Ca2+-dependent biofilm development in sea water
复制标题
DOI：
10.1073/pnas.2334614100
复制
发表时间：
2003-11-25
影响因子：
11.1
通讯作者：
Watnick, PI
中科院分区：
文献类型：
--
作者：
Kierek, K;Watnick, PI
霍乱弧菌是河口环境中的一种寄生菌，也是引起霍乱的病原体。先前的工作已经证明，霍乱弧菌形成胞外多糖依赖性生物膜和Ca 2+依赖性生物膜。在这项工作中，我们证明了O-抗原多糖的霍乱弧菌在钙依赖的生物膜的发展模型和真正的海水中的作用。有趣的是，霍乱弧菌生物膜，以及其他几种弧菌的生物膜，当Ca 2+从浴介质中除去时，解体，表明Ca 2+直接与O-抗原多糖相互作用。在孟加拉湾，霍乱发病率与海面高度增加有关。由于该区域海拔较低，海面高度的增加可能导致海水、海洋颗粒物和海洋生物膜进入淡水环境。由于淡水是Ca 2+贫乏，我们的研究结果表明，增加的一个潜在结果是海面高度是海洋生物膜的分散，伴随着海洋细菌如霍乱弧菌的增加。这种现象可能有助于增加海面高度与霍乱的相关性。
Vibrio cholerae is both an inhabitant of estuarine environments and the etiologic agent of the diarrheal disease cholera. Previous work has demonstrated that V. cholerae forms both an exopolysaccharide-dependent biofilm and a Ca2+-dependent biofilm. In this work, we demonstrate a role for the O-antigen polysaccharide of V. cholerae in Ca2+-dependent biofilm development in model and true sea water. Interestingly, V. cholerae biofilms, as well as the biofilms of several other Vibriospecies, disintegrate when Ca2+ is removed from the bathing medium, suggesting that Ca2+ is interacting directly with the O-antigen polysaccharide. In the Bay of Bengal, cholera incidence has been correlated with increased sea surface height. Because of the low altitude of this region, increases in sea surface height are likely to lead to transport of sea water, marine particulates, and marine biofilms into fresh water environments. Because fresh water is Ca2+-poor, our results suggest that one potential outcome of an increase is sea surface height is the dispersal of marine biofilms with an attendant increase in planktonic marine bacteria such as V. cholerae. Such a phenomenon may contribute to the correlation of increased sea surface height with cholera.