TISSUE-SPECIFIC EXPRESSION OF THE RI AND RII SODIUM-CHANNEL SUBTYPES
TISSUE-SPECIFIC EXPRESSION OF THE RI AND RII SODIUM-CHANNEL SUBTYPES
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DOI：
10.1073/pnas.84.23.8682
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发表时间：
1987-12-01
影响因子：
11.1
通讯作者：
CATTERALL, WA
中科院分区：
文献类型：
--
作者：
GORDON, D;MERRICK, D;CATTERALL, WA
制备区分大鼠脑钠通道亚型（称为RI和RII）的抗肽抗体，并用于测定其在神经和肌肉组织中的相对表达。从大鼠脑中纯化的钠通道约为18%RI和80%RII。在脑中，RII亚型优先表达，其中RI/RII比率范围从海马中的0.07到大脑皮层中的0.17。RI亚型优先在中枢神经系统的更尾部区域表达，其中延髓的RI/RII值为0.98，脊髓为2.2。在中脑、髓质和脊髓中检测到另外的未鉴定的钠通道亚型的表达，并且在视网膜和视神经中未鉴定的钠通道亚型的表达超过RI和RII的表达。RI和RII亚型主要在中枢神经系统中表达，在骨骼肌或心肌、交感神经节、肾上腺髓质、坐骨神经或马尾中未检测到显著数量。RII亚型首先出现在脑和脊髓的发育中，但随着RI亚型的增加，在成年脊髓中出现下降。这两种钠通道亚型的严格区域表达表明它们可能具有不同的功能特性或生理作用。
Anti-peptide antibodies that distinguish between the rat brain sodium channel subtypes referred to as RI and RII were prepared and used to determine their relative expression in nerve and muscle tissues. Sodium channels purified from rat brain are approximately 18% RI and 80% RII. In brain, the RII subtype is preferentially expressed with RI/RII ratios ranging from 0.07 in the hippocampus to 0.17 in the cerebral cortex. The RI subtype is preferentially expressed in more caudal areas of the central nervous system with values of RI/RII of 0.98 for medulla oblongata and 2.2 for spinal cord. Expression of additional unidentified sodium channel subtype(s) is detected in midbrain, medulla, and spinal cord, and expression of unidentified sodium channel subtypes predominates over expression of RI and RII in retina and optic nerve. The RI and RII subtypes are primarily expressed in the central nervous system and are not detected in significant numbers in skeletal or cardiac muscle, sympathetic ganglia, adrenal medulla, sciatic nerve, or cauda equina. The RII subtype appears first in development of both brain and spinal cord but declines in adult spinal cord as the RI subtype increases. The strict regional expression of these two sodium channel subtypes suggests that they may have distinct functional properties or physiological roles.