Linseed Oil in the Maternal Diet during Gestation and Lactation Modifies Fatty Acid Composition, Mucosal Architecture, and Mast Cell Regulation of the Ileal Barrier in Piglets
Linseed Oil in the Maternal Diet during Gestation and Lactation Modifies Fatty Acid Composition, Mucosal Architecture, and Mast Cell Regulation of the Ileal Barrier in Piglets
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DOI：
10.3945/jn.108.102640
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发表时间：
2009-06-01
影响因子：
4.2
通讯作者：
Le Huerou-Luron, Isabelle
中科院分区：
文献类型：
--
作者：
Boudry, Gaelle;Douard, Veronique;Le Huerou-Luron, Isabelle
在这项研究中，我们研究了在仔猪回肠中添加亚麻籽油（富含18：3（n-3））对脂肪酸组成、粘膜结构和肥大细胞调节屏障功能的影响。16头母猪在妊娠期和哺乳期饲喂猪油（LAR）或亚麻籽油（LSO）为基础的饲料。测定了分娩时母体红细胞和泌乳第14天乳汁的脂肪酸组成。脂肪酸组成，绒毛隐窝结构，辣根过氧化物酶的渗透性，肥大细胞脱粒后，在乌辛室测定仔猪回肠在D 0，7，和28。在第0天，LSO组的母体RBC和仔猪回肠中18：3（n-3）和20：5（n-3）水平较高，而22：6（n-3）和20：4（n-6）水平较低。LSO母猪乳汁中18：3（n-3）水平也较高。在第7天和第28天，LSO仔猪回肠中18：3（n-3）的水平较高。此外，在第28天，20：4（n-6），LSO中的回肠水平倾向于（P = 0.09）低于LAR仔猪，与Delta 5去饱和酶的mRNA表达较低平行。与LAR仔猪相比，LSO仔猪在第0天绒毛较短，在第7天隐窝较短。肥大细胞脱颗粒对回肠通透性的影响随年龄的增长而降低，但在LSO组（第7天）比LAR组（第28天）更快达到最低值。总之，母亲饮食中添加亚麻籽油深刻地改变了后代回肠的脂肪酸组成、结构和生理学。J.营养139：1110-1117，2009.
In this study, we investigated the effect of supplementation of the maternal diet with linseed oil [rich in 18:3(n-3)) on fatty acid composition, mucosal architecture, and mast cell regulation of barrier function in piglet ileum. Sixteen sows were fed a lard LAR)- or a linseed oil (LSO)-based diet during gestation and lactation. Fatty acid composition of maternal RBC at parturition and of milk at d 14 of lactation were determined. Fatty acid composition, villous-crypt structure, and permeability to horseradish peroxidase in Ussing chambers after mast cell degranulation were determined in the ileum of piglets at d 0, 7, and 28. At d 0, 18:3(n-3) and 20:5(n-3) levels were higher, but 22:6(n-3) and 20:4(n-6) levels were lower in both maternal RBC and piglet ileum of the LSO group. Levels of 18:3(n-3) were also higher in the milk of LSO sows. Levels of 18:3(n-3) were higher in LSO piglet ileum at d 7 and 28. Moreover, at d 28, 20:4(n-6) ileal levels tended (P = 0.09) to be lower in LSO than in LAR piglets, in parallel with a lower mRNA expression of Delta 5 desaturase. LSO piglets had shorter villi at d 0 and shorter crypts at d 7 compared with LAR piglets. The effect of mast cell degranulation on ileal permeability decreased with age in both groups but reached a minimum sooner in the LSO group (d 7) than in the LAR group (d 28). In conclusion, linseed oil supplementation of the maternal diet profoundly modifies the fatty acid composition, structure, and physiology of the offspring ileum. J. Nutr. 139: 1110-1117, 2009.