Priming of the respiratory burst of neutrophils by diacylglycerol. Independence from activation or translocation of protein kinase C.
Priming of the respiratory burst of neutrophils by diacylglycerol. Independence from activation or translocation of protein kinase C.
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二酰甘油启动中性粒细胞的呼吸爆发。
DOI：
10.1016/s0021-9258(18)48290-7
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
L. Mcphail
中科院分区：
文献类型：
--
作者：
D. Bass;C. Gerard;P. Olbrantz;J. Wilson;C. McCall;L. Mcphail
中性粒细胞与某些激动剂的预孵育可能“启动”细胞，引起对第二刺激（“启动刺激”）的反应增加。我们使用了两种方法来研究蛋白激酶C （Ca2+/磷脂依赖酶）在1-油基-2-乙酰甘油（OAG）、12-肉豆酸酯13-醋酸酯（PMA）和n -甲酰- met - leu - phe （fMLP）的启动和刺激中的作用：1-（5-异喹啉磺酰）-哌嗪（C- i）对蛋白激酶C的抑制作用，以及OAG的启动和刺激浓度诱导的蛋白激酶C易位的测量。C-I对fMLP的刺激或启动刺激几乎没有影响，这表明fMLP调用独立于蛋白激酶c的事件，C-I同样抑制PMA的刺激和启动刺激。OAG的直接刺激被抑制，而OAG的启动和被启动刺激未受C-I的影响。OAG浓度大于或等于100微米可引起蛋白激酶C易位，与直接刺激呼吸爆发有关。较低的OAG浓度（10-30微米）引发了fMLP的刺激，相反，刺激了fMLP引发的中性粒细胞，但没有引起蛋白激酶C的易位。这些数据与先前的假设一致，即PMA和OAG通过蛋白激酶C的易位和激活直接刺激多形核中性粒细胞。然而，OAG的启动和引发的刺激显然引发了不同的转导机制，而不是蛋白激酶C易位。
Preincubation of neutrophils with certain agonists may “prime” the cells to cause increased responses to a second stimulus (“primed stimulation”). We used two approaches to examine the role of protein kinase C (Ca2+/phospholipid-dependent enzyme) in priming and stimulation by 1-oleoyl-2-acetylglycerol (OAG), phorbol 12-myristate 13-acetate (PMA), and N-formyl-Met-Leu-Phe (fMLP): inhibition of protein kinase C by 1-(5-isoquinolinesulfonyl)-piperazine (C-I) and measurement of protein kinase C translocation induced by priming and stimulatory concentrations of OAG. C-I had little effect on stimulation or primed stimulation by fMLP, suggesting that fMLP invokes events independent of protein kinase C. C-I equally inhibited stimulation and primed stimulation by PMA. Direct stimulation by OAG was inhibited, but priming and primed stimulation by OAG was unaltered by C-I. OAG concentrations greater than or equal to 100 microM caused translocation of protein kinase C, in correlation with direct stimulation of the respiratory burst. Lower OAG concentrations (10-30 microM) primed to stimulation by fMLP and, conversely, stimulated neutrophils primed with fMLP, yet did not cause translocation of protein kinase C. The data are compatible with previous assumptions that PMA and OAG directly stimulate polymorphonuclear neutrophil leukocytes by translocation and activation of protein kinase C. However, priming and primed stimulation by OAG apparently invoke distinct transduction mechanisms other than protein kinase C translocation.
DOI：
10.1016/s0021-9258(18)34459-4
发表时间：
1982-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
M. Castagna;Y. Takai;K. Kaibuchi;K. Sano;U. Kikkawa;Y. Nishizuka
通讯作者：
M. Castagna;Y. Takai;K. Kaibuchi;K. Sano;U. Kikkawa;Y. Nishizuka
DOI：
--
发表时间：
1980-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
A. Kishimoto;Y. Takai;T. Mori;U. Kikkawa;Y. Nishizuka
通讯作者：
A. Kishimoto;Y. Takai;T. Mori;U. Kikkawa;Y. Nishizuka
花生四烯酸刺激人中性粒细胞释放超氧化物机制的研究。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Curnutte,JT;Badwey,JA;Robinson,JM;Karnovsky,MJ;Karnovsky,ML
通讯作者：
Karnovsky,ML