ISOLATION AND LONG-TERM CELL CULTURE OF EPITHELIAL-LIKE CELLS FROM RAT LIVER
ISOLATION AND LONG-TERM CELL CULTURE OF EPITHELIAL-LIKE CELLS FROM RAT LIVER
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DOI：
10.1016/0014-4827(71)90316-8
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发表时间：
1971-01-01
影响因子：
3.7
通讯作者：
WEISBURGER, JH
中科院分区：
文献类型：
--
作者：
WILLIAMS, GM;WEISBURGER, EK;WEISBURGER, JH
上皮样细胞从肝脏的10日龄大鼠分离成纤维细胞样细胞的基础上的差异附着到培养表面。从上皮样细胞的培养物中进一步消除持续存在的成纤维细胞样细胞后，启动后一种细胞类型的细胞系，这些细胞系在长达9个月的连续培养中保持其形态，并且在注射到同基因宿主中时不显示致瘤性。
Epithelial-like cells from livers of 10-day-old rats were separated from fibroblast-like cells on the basis of differential attachment to a culture surface. After further elimination of persisting fibroblast-like cells from the cultures of epithelial-like cells, lines of the latter cell type were initiated and these maintained their morphology for up to nine months of continuous culture and displayed no tumorigenicity upon injection into syngeneic hosts.