Aerobic and anaerobic metabolism in a facultative anaerobe Ep01 lacking cytochrome, quinone and catalase
Aerobic and anaerobic metabolism in a facultative anaerobe Ep01 lacking cytochrome, quinone and catalase
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缺乏细胞色素、醌和过氧化氢酶的兼性厌氧菌 Ep01 的需氧和厌氧代谢
DOI：
10.1111/j.1574-6968.1989.tb03556.x
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发表时间：
1989
影响因子：
2.1
通讯作者：
M. Kozaki
中科院分区：
文献类型：
--
作者：
Y. Niimura;E. Koh;T. Uchimura;Naohiro Ohara;M. Kozaki
兼性厌氧菌EP01厌氧发酵葡萄糖产生丙酮酸、甲酸盐、乙酸乙酯和乙醇的混合物，厌氧产乙酸丙酮酸和丙酮酸。厌氧培养细胞的细胞提取物含有活性的丙酮酸甲酸水解酶、乙醛脱氢酶和乙醇脱氢酶，而有氧培养的细胞提取物含有活性的丙酮酸脱氢酶系统、NADH氧化酶和NADH过氧化物酶。在这两种条件下生长的细胞中，醋酸激酶和磷酸乙酰转移酶的活性水平没有显著差异。根据代谢产物和酶活性，我们提出菌株EP01存在两条代谢途径，即在厌氧条件下形成甲酸盐、乙酸盐和乙醇的途径和在好氧条件下形成的途径。这就解释了为什么菌株Ep01在严格的厌氧条件和充气条件下都能很好地生长。
A facultative anaerobe, strain Ep01 produced a mixture of pyruvate, formate, acetate and ethanol from glucose anaerobically, and acetate and pyruvate aerobically. Cell extract of anaerobic-grown cells contained active pyruvate formatelyase, aldehyde dehydrogenase and alcohol dehydrogenase, while cell extract of aerobic grown cells contained an active pyruvate dehydrogenase system, NaDH oxidase and NADH peroxidase. Levels of acetate kinase and phosphate acetyltransferase activities were not significantly different in cells grown under either condition. Based on the metabolic products and the emzyme activities, we propose the presence of two metabolic pathways in strain Ep01, namely, a pathway to form formate, acetate and ethanol under anaerobic conditions, and a pathway to form under aerobic conditions. This explains why strain Ep01 can grow well both under strictly anaerobic conditions and well-aerated conditions.