An integrated process for enzymatic catalysis allowing product recovery and enzyme reuse by applying thermoreversible aqueous biphasic systems.
An integrated process for enzymatic catalysis allowing product recovery and enzyme reuse by applying thermoreversible aqueous biphasic systems.
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DOI：
10.1039/c7gc03880a
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发表时间：
2018-03-21
期刊：
影响因子：
--
通讯作者：
Coutinho JAP
中科院分区：
文献类型：
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作者：
Ferreira AM;Passos H;Okafuji A;Tavares APM;Ohno H;Freire MG;Coutinho JAP
热可逆水双相体系（ABS）组成的氨基两性离子（ZIs）和聚合物在这里公开作为集成的生物反应分离过程。涉及漆酶的生物催化反应发生在均质介质中，之后温度的微小变化诱导两相的形成，酶与产物在一步中完全分离。这些系统还允许酶的回收和再利用，以及富锌相，有助于可持续生物催化过程的发展。
Thermoreversible aqueous biphasic systems (ABS) composed of ammonium-based zwitterions (ZIs) and polymers are here disclosed to act as integrated bioreaction-separation processes. The biocatalytic reaction involving laccase occurs in homogeneous media, after which small changes in temperature induce the formation of two phases and the complete separation of the enzyme from the products in a single-step. These systems also allow the recover and reuse of the enzyme, along with the ZI-rich phase, contributing towards the development of sustainable biocatalytic processes.