MURINE MYCOPLASMA RESPIRATORY-DISEASE
MURINE MYCOPLASMA RESPIRATORY-DISEASE
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DOI：
10.1111/j.1749-6632.1973.tb45665.x
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发表时间：
1973-01-01
期刊：
影响因子：
--
通讯作者：
BAKER, HJ
中科院分区：
文献类型：
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作者：
CASSELL, GH;LINDSEY, JR;BAKER, HJ
与许多栖息于哺乳动物呼吸道并作为病原体的作用可疑的支原体不同，4l M。已知肺结核对小鼠具有高致病性。我们实验室最近的研究已经确定了鼻内M强毒株剂量之间的定量关系。肺结核及其在小鼠中引起的疾病。接种剂量被认为是疾病的严重程度，持续时间和病理特征的主要决定因素。无病原体（PF）t小鼠鼻内接种M.肺杆菌，剂量范围为10 - 9 × 10 '-9菌落形成单位（CFU）。临床疾病和死亡仅发生在给予lo 5或更高CFU的动物中。疾病的外在症状基本上与Lutsky和Organik报道的相同，除了我们偶尔在一只动物身上观察到“喋喋不休”。死亡特征性地遵循双峰分布，如图1所示。组织学检查显示，第一和第二个死亡高峰分别归因于主要涉及肺泡和支气管的病理事件。
Unlike many mycoplasmas that inhabit the respiratory tracts of mammals and have questionable roles as pathogen~,~~, 4l M. pulmonis is known to be highly pathogenic for mice. s9 Recent studies3s in our laboratory have defined the quantitative relationship between intranasal dose of a virulent strain of M. pulmonis and the disease it causes in mice. Inoculation dose was found to be the major determinant of severity duration, and pathologic character of the disease. Pathogen-free (PF) t mice were inoculated intranasally with broth cultures of M. pulmonis in doses ranging from 10’-9 X lo9 colony-forming units (CFU). Clinical illness and deaths occurred only in animals given lo5 or greater CFU. The outward signs of illness were essentially the same as those reported by Lutsky and Organi~ k,~~ except that we observed “chattering” in an occasional animal. Deaths characteristically followed a bimodal distribution as illustrated in FIGURE 1. Histologic examinations revealed that the first and second peaks of death were attributable to pathologic events involving primarily alveoli and bronchi, respectively.