Mechanism of DNA replication‐dependent transcriptional activation of the acetylcholinesterase gene in the Ciona intestinalis embryo
Mechanism of DNA replication‐dependent transcriptional activation of the acetylcholinesterase gene in the Ciona intestinalis embryo
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玻璃海鞘胚胎中乙酰胆碱酯酶基因 DNA 复制依赖性转录激活机制
DOI：
10.1111/j.1440-169x.2009.01147.x
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发表时间：
2009
期刊：
影响因子：
4.6
通讯作者：
S. Fujiwara
中科院分区：
文献类型：
--
作者：
Y. Kataoka;Ryo Mishina;S. Fujiwara
海鞘中的乙酰胆碱酯酶编码基因（Ci-AChE）在原肠胚阶段的尾肌细胞中表达。当胚胎从32细胞期开始连续用阿非迪霉素处理时，即使当对照胚胎达到尾芽期时，Ci‐AChE也不表达。这一结果表明，Ci-AChE在经过一定数量的DNA复制循环后获得转录能力。含有Ci-AChE 5′侧翼区的lacZ报告基因在尾肌细胞中表达。从32细胞期开始的阿非迪霉素处理影响但不完全抑制lacZ的表达。亚硫酸氢盐测序分析5′侧翼序列和第一外显子内的4个区域的甲基化状态。然而，从16细胞到110细胞阶段，所有这些区域都保持未甲基化。结果表明，Ci-AChE基因座的DNA不负责计数复制的轮次。我们检查了C. Escherichia coli MyoD（Ci-MyoD）的表达，这是一种激活Ci-AChE的转录因子。 从32细胞阶段开始的阿非迪霉素处理抑制了Ci‐MyoD的表达，即使当对照胚胎达到原肠胚阶段时也是如此。这些结果表明，缺乏Ci‐MyoD对阿非迪霉素处理的胚胎中Ci‐AChE的抑制至关重要。
The acetylcholinesterase‐encoding gene in the ascidian Ciona intestinalis (Ci‐AChE) is expressed in tail muscle cells from the gastrula stage. When the embryo was continuously treated with aphidicolin from the 32‐cell stage, Ci‐AChE was not expressed even when control embryos reached the tailbud stage. This result suggests that Ci‐AChE acquires the competence to be transcribed after passing through a certain number of DNA replication cycles. A lacZ reporter gene containing the 5′ flanking region of Ci‐AChE was expressed in the tail muscle cells. Aphidicolin treatment from the 32‐cell stage affected, but did not completely suppress, the expression of lacZ. A bisulfite sequencing analysis was carried out to examine the methylation status of four regions within the 5′ flanking sequence and the first exon. However, all of these regions remained unmethylated from the 16‐cell to 110‐cell stages. The results suggested that the DNA of the Ci‐AChE locus is not responsible for counting the rounds of replication. We examined the expression of the C. intestinalis MyoD (Ci‐MyoD), a transcription factor that activates Ci‐AChE. Aphidicolin treatment from the 32‐cell stage suppressed the expression of Ci‐MyoD, even when control embryos reached the gastrula stage. These results suggest that a lack of Ci‐MyoD is critical to the suppression of Ci‐AChE in aphidicolin‐treated embryos.
影响因子：
2.7
作者：
Meedel, Thomas H.;Chang, Patrick;Yasuo, Hitoyoshi
通讯作者：
Yasuo, Hitoyoshi