Transcription, nucleosome positioning and protein binding modulate nucleotide excision repair of the Saccharomyces cerevisiae MET17 promoter
Transcription, nucleosome positioning and protein binding modulate nucleotide excision repair of the Saccharomyces cerevisiae MET17 promoter
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DOI：
10.1016/s1568-7864(02)00239-2
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发表时间：
2003-04-02
期刊：
影响因子：
3.8
通讯作者：
Waters, R
中科院分区：
文献类型：
--
作者：
Powell, NG;Ferreiro, J;Waters, R
我们已经评估了转录，染色质结构和蛋白结合如何调节内源性酿酒酵母基因MET 17的上游调控区和早期编码区的核苷酸切除修复，从这些区域测量UV诱导的环丁烷嘧啶二聚体的去除，其中转录和染色质结构可以相互独立地调节。明显的修复趋势取决于转录状态。当转录被抑制时，核小体定位和蛋白质结合通过染色质免疫沉淀和定量实时PCR确定，是重要的因素。核小体定位和/或蛋白质结合效应在转录的链上最明显，修复在无核小体区域发生最快，但在该区域内结合调节蛋白的地方被阻滞。当转录被去抑制时，在TATA盒上游200 bp开始并向下游延伸到编码区的区域中，两条链的修复速率都增加。这种效应超过了核小体定位和蛋白质结合的影响。(C)2002 Elsevier Science B. V.保留所有权利。
We have assessed how transcription, chromatin structure and protein binding modulate nucleotide excision repair in the upstream regulatory region and early coding region of the endogenous Saccharomyces cerevisiae gene MET17 Removal of UV-induced cyclobutane pyrimidine dimers was measured from these regions, in which transcription and chromatin structure could be regulated independently of each other. Distinct repair trends were apparent depending on transcriptional state., When transcription was repressed nucleosome positioning and protein binding as determined by chromatin immunoprecipitation and quantitative real-time PCR, were significant factors. Nucleosome positioning and/or protein binding effects were most apparent on the strand that becomes transcribed, with repair occurring fastest in a nucleosome free region but being retarded where regulatory proteins bound within this region. When transcription was derepressed the rate of repair increased on both strands in a region beginning 200 bp upstream of the TATA box and extending downstream into the coding region. This effect overrode the influences of nucleosome positioning and protein binding. (C) 2002 Elsevier Science B.V. All rights reserved.