Cytokine mRNA expression in the labial salivary gland tissues from patients with primary Sjögren's syndrome.
Cytokine mRNA expression in the labial salivary gland tissues from patients with primary Sjögren's syndrome.
复制标题
原发性干燥综合征患者唇唾液腺组织中细胞因子 mRNA 的表达。
DOI：
10.1093/rheumatology/34.4.326
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
H. Moutsopoulos
中科院分区：
文献类型：
--
作者：
D. Boumba;F. Skopouli;H. Moutsopoulos
采用原位杂交技术和白细胞介素-1 β寡核苷酸探针检测原发性干燥综合征（pSS）患者（n = 12）和对照组（n = 8）冷冻小涎腺组织中细胞因子mRNA的表达模式（IL-1 β）、肿瘤坏死因子α和β（TNF-α和TNF-β），白细胞介素-6（IL-6），白细胞介素-2（IL-2）及其受体（IL-2 R），白细胞介素-4（IL-4），白细胞介素-10（IL-10），检测干扰素-γ（IFN-γ）和转化生长因子-β（TGF-β）。除了原位杂交，免疫组化被用来确定表达IL-2和IL-4 mRNA的细胞的子集。发现pSS患者的小唾液腺病变中涉及的单核细胞表达促炎细胞因子如TNF-α和IL-1 β以及参与B细胞和T细胞功能调节的细胞因子（IL-2和IL-6）的mRNA。相比之下，只有三个pSS患者的活检表达抑制性细胞因子，如IFN-γ和TGF-β的mRNA。此外，IL-6和IL-1 β的mRNA也在腺上皮细胞中检测到，提示这些细胞可能在干燥综合征的自身免疫性病变的发病机制中起作用。IL-10 mRNA未检测到，而IL-4 mRNA主要在轻度和早期病变患者的幼稚T淋巴细胞中检测到。这些结果表明，单核细胞和上皮细胞的细胞因子的本地生产可能参与免疫介导的破坏外分泌腺的pSS患者。
The pattern of cytokine mRNA expression in frozen minor salivary gland tissues from patients with primary Sjögren's syndrome (pSS) (n = 12) and controls (n = 8) using an in situ hybridization technique and oligonucleotide probes of interleukin-1 beta (IL-1 beta), tumour necrosis factor alpha and beta (TNF-alpha and TNF-beta), interleukin-6 (IL-6), interleukin-2 (IL-2) and its receptor (IL-2R), interleukin-4 (IL-4), interleukin-10 (IL-10), interferon-gamma (IFN-gamma) and transforming growth factor-beta (TGF-beta) was examined. In addition to in situ hybridization, immunohistochemistry was used to identify the subset of cells expressing IL-2 and IL-4 mRNA. Mononuclear cells involved in the minor salivary gland lesions of pSS patients were found to express mRNA for pro-inflammatory cytokines such as TNF-alpha and IL-1 beta, and cytokines involved in the regulation of B- and T-cell function (IL-2 and IL-6). In contrast, only three biopsies from patients with pSS express mRNA of inhibitory cytokines such as IFN-gamma and TGF-beta. Furthermore mRNA for IL-6 and IL-1 beta was also detected in the glandular epithelial cells suggesting that these cells may play a role in the pathogenesis of autoimmune lesion in Sjögren's syndrome. IL-10 mRNA was not detected while IL-4 mRNA was primarily detected in naïve T-lymphocytes of patients with a mild and early lesion. These results suggest that local production of cytokines by both mononuclear and epithelial cells may be involved in the immune-mediated destruction of exocrine glands in patients with pSS.