Monoclonal antibodies recognize individual neurofilament triplet proteins.
Monoclonal antibodies recognize individual neurofilament triplet proteins.
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单克隆抗体可识别单个神经丝三联蛋白。
DOI：
10.1073/pnas.79.19.6089
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发表时间：
1982
影响因子：
11.1
通讯作者：
Schlaepfer,WW
中科院分区：
文献类型：
--
作者：
Lee,V;Wu,HL;Schlaepfer,WW
制备了12株抗牛神经丝蛋白(NF三联体蛋白，分别命名为P68、P150和P200)的单抗。其中六种抗体识别单个核因子亚基上的独特决定因素。其他6个被认为是至少两个核因子三联体蛋白的共同决定因素。其中六种抗体识别分离的、固定在硝酸纤维素纸中的个体神经纤维素酶。通过过氧化物酶-抗过氧化物酶技术，所有抗体都染色神经元及其突起，其方式与众所周知的中枢神经系统和外周神经系统中神经营养因子的分布相同。这些结果有力地表明，NF亚单位是不同但相关的蛋白质，较小的NF亚单位(P68和P150)不是来自较大的NF蛋白。
A series of 12 monoclonal antibodies has been prepared against crude or purified individual bovine neurofilament (NF) triplet proteins (designated as P68, P150, and P200). Six of the antibodies recognized unique determinants on individual NF subunits. The other six recognized shared determinants on at least two NF triplet proteins. Six of the antibodies recognized separated, immobilized individual NF in nitrocellulose paper. By the peroxidase-antiperoxidase technique, all of the antibodies stained neurons and their processes in a manner identical to the well-known distribution of NF in both the central and peripheral nervous system. These results strongly suggest that the NF subunits are distinct but related proteins and that the smaller NF subunits (P68 and P150) are not derived from larger NF proteins.