CD27(hi)CD38(hi) plasmablasts are activated B cells of mixed origin with distinct function.
CD27(hi)CD38(hi) plasmablasts are activated B cells of mixed origin with distinct function.
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DOI：
10.1016/j.isci.2021.102482
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发表时间：
2021-05-21
期刊：
影响因子：
5.8
通讯作者：
Fink K
中科院分区：
文献类型：
--
作者：
Rouers A;Appanna R;Chevrier M;Lum J;Lau MC;Tan L;Loy T;Tay A;Sethi R;Sathiakumar D;Kaur K;Böhme J;Leo YS;Renia L;Howland SW;Singhal A;Chen J;Fink K
在多次感染过程中，具有临床重要性的广泛反应性B细胞会发生演变，二次感染后重新激活的B细胞与初次感染后激活的B细胞不同。在此，我们研究了初次或二次登革热病毒感染患者的CD27高表达CD38高表达浆母细胞。确定了三个在转录和功能上不同的细胞群。最大的0/1群与浆细胞相关，细胞编码IgG1同种型的血清型交叉反应抗体，这与在滤泡外反应期间记忆B细胞的激活一致。2群和3群的细胞表达低水平的抗体基因以及高水平的与氧化磷酸化、EIF2通路和线粒体功能障碍相关的基因。2群和3群显示出T细胞辅助的转录特征，符合从初始B细胞或记忆B细胞激活的情况。我们的研究结果有助于理解人类在自然初次和二次感染后发生的平行B细胞激活事件。
登革热患者的CD27高表达CD38高表达浆母细胞形成三个细胞群
0/1群表达高水平且更具登革热特异性的抗体
0/1群让人联想到滤泡外激活
2群和3群代谢活跃，具有T细胞辅助的表达特征
免疫学；细胞生物学；细胞生物学的功能方面；系统生物学
Clinically important broadly reactive B cells evolve during multiple infections, with B cells re-activated after secondary infection differing from B cells activated after a primary infection. Here we studied CD27highCD38high plasmablasts from patients with a primary or secondary dengue virus infection. Three transcriptionally and functionally distinct clusters were identified. The largest cluster 0/1 was plasma cell-related, with cells coding for serotype cross-reactive antibodies of the IgG1 isotype, consistent with memory B cell activation during an extrafollicular response. Cells in clusters 2 and 3 expressed low levels of antibody genes and high levels of genes associated with oxidative phosphorylation, EIF2 pathway, and mitochondrial dysfunction. Clusters 2 and 3 showed a transcriptional footprint of T cell help, in line with activation from naive B cells or memory B cells. Our results contribute to the understanding of the parallel B cell activation events that occur in humans after natural primary and secondary infection. CD27highCD38high plasmablasts from patients with dengue form three clusters Clusters 0/1 express high levels, and more dengue-specific, antibodies Clusters 0/1 are reminiscent of extrafollicular activation Clusters 2 and 3 are metabolically active with an expression footprint of T cell help Immunology; Cell biology; Functional aspects of cell biology; Systems biology
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影响因子：
11.1
作者：
Fibriansah, Guntur;Tan, Joanne L.;Smith, Scott A.;de Alwis, Adamberage R.;Thiam-Seng Ng;Kostyuchenko, Victor A.;Ibarra, Kristie D.;Wang, Jiaqi;Harris, Eva;de Silva, Aravinda;Crowe, James E., Jr.;Lok, Shee-Mei
通讯作者：
Lok, Shee-Mei
影响因子：
7
作者：
Harrow J;Frankish A;Gonzalez JM;Tapanari E;Diekhans M;Kokocinski F;Aken BL;Barrell D;Zadissa A;Searle S;Barnes I;Bignell A;Boychenko V;Hunt T;Kay M;Mukherjee G;Rajan J;Despacio-Reyes G;Saunders G;Steward C;Harte R;Lin M;Howald C;Tanzer A;Derrien T;Chrast J;Walters N;Balasubramanian S;Pei B;Tress M;Rodriguez JM;Ezkurdia I;van Baren J;Brent M;Haussler D;Kellis M;Valencia A;Reymond A;Gerstein M;Guigó R;Hubbard TJ
通讯作者：
Hubbard TJ
影响因子：
20.3
作者：
Basso, K;Klein, U;Dalla-Favera, R
通讯作者：
Dalla-Favera, R
DOI：
10.1093/bioinformatics/btw631
发表时间：
2017-02-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Canzar S;Neu KE;Tang Q;Wilson PC;Khan AA
通讯作者：
Khan AA
影响因子：
5.4
作者：
Angelo, Michael A.;Grifoni, Alba;Weiskopf, Daniela
通讯作者：
Weiskopf, Daniela