Xbal polymorphism of apolipoprotein B gene in a Tunisian population: alleles frequencies and relationship with plasma lipid parameters
Xbal polymorphism of apolipoprotein B gene in a Tunisian population: alleles frequencies and relationship with plasma lipid parameters
复制标题
DOI：
10.1684/abc.2007.0127
复制
发表时间：
2007-05-01
影响因子：
0.5
通讯作者：
Kaabachi, N.
中科院分区：
文献类型：
--
作者：
Kallel, A.;Jemaa, R.;Kaabachi, N.
载脂蛋白B（Apo B）是乳糜微粒、低密度脂蛋白（LDL）、极低密度脂蛋白（VLDL）和中密度脂蛋白（IDL）的组分，是LDL受体的配体。因此，载脂蛋白B在脂蛋白代谢和维持血清胆固醇水平的正常稳态中起核心作用。据报道，几种载脂蛋白B限制性片段长度多态性（XbaI、EcoRI、MspI）与血脂水平变化、肥胖和/或冠状动脉疾病相关。到目前为止，没有数据可用于XbaI载脂蛋白B多态性和血脂水平之间的关系在突尼斯人口。在这里，我们报告的载脂蛋白B基因的XbaI多态性的频率，我们评估这种多态性对突尼斯人口的脂质和脂蛋白浓度的影响。对296名突尼斯人（112名女性和184名男性，年龄51.4 ± 9.6岁）的血液样本进行了总胆固醇、甘油三酯、HDL-胆固醇和载脂蛋白Al和B分析。同时，通过聚合酶链反应（PCR）和限制性片段长度多态性（RFLP）进行基因分型。XbaI多态性与血浆胆固醇（p = 0.04）、甘油三酯（p = 0.02）和载脂蛋白A I（p = 0.004）的差异相关，具有基因型X1 X1的个体具有最低的平均水平，具有基因型X2 X2的个体具有最高的平均水平，对于该多态性杂合的个体具有中间水平。根据性别，XbaI多态性效应仅在男性甘油三酯中观察到。因此，结果表明，载脂蛋白B基因的XbaI多态性对血清总胆固醇，甘油三酯和载脂蛋白A1浓度在突尼斯人口的影响。
Apolipoprotein B (Apo B) is a component of chylomicrons, low-density lipoproteins (LDL), very low density lipoproteins (VLDL), and intermediate-density lipoproteins (IDL) and is the ligand for the LDL receptor. Thereby, Apo B plays a central role in lipoprotein metabolism and in maintaining the normal homeostasis of serum cholesterol levels. Several Apo B restriction fragment length polymorphisms (XbaI, EcoRI, MspI) have been reported to be associated with variation in lipid levels, obesity and/or coronary artery disease. To date, no data are available on relationship between XbaI Apo B polymorphism and lipid levels in Tunisian population. Here, we report frequencies of the XbaI polymorphism of the Apo B gene and we assess the effect of this polymorphism on lipid and lipoprotein concentrations in Tunisian population. Blood samples from 296 Tunisian individuals (112 women and 184 men, aged 51.4 +/- 9.6 years), were analysed for total cholesterol, triglycerides, HDL-cholesterol and apolipoproteins Al and B. In parallel, genotyping by means of polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) was performed. The XbaI polymorphism was associated with differences in plasma cholesterol (p = 0.04), triglyceride (p = 0.02) and apolipoprotein A I (p = 0.004), individuals with the genotype X1X1 have the lowest mean levels and those with the genotype X2X2 have the highest, with the individuals heterozygous for the polymorphism having intermediate levels. According to sex, the XbaI polymorphism effect was only observed for triglyceride in men. Thus, the results demonstrate an influence of XbaI polymorphism of Apo B gene on se rum total-cholesterol, triglycerides and apolipoprotein A1 concentrations among Tunisian population.