Polarized, Amelogenin Expressing Ameloblast-Like Cells from Cervical Loop/Dental Pulp Cocultures in Bioreactors.
Polarized, Amelogenin Expressing Ameloblast-Like Cells from Cervical Loop/Dental Pulp Cocultures in Bioreactors.
复制标题
来自生物反应器中颈环/牙髓共培养物的极化、表达釉原素的成釉细胞样细胞。
DOI：
10.1089/scd.2021.0115
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发表时间：
2021
影响因子：
4
通讯作者：
Diekwisch,ThomasGH
中科院分区：
文献类型：
--
作者：
Pandya,Mirali;Lyu,Huling;Luan,Xianghong;Diekwisch,ThomasGH
长的、极化的成釉细胞样细胞的生长一直被认为是釉质组织工程的主要先决条件。在这项研究中，我们设计了三维生物反应器/支架微环境来增殖和评估宫颈环衍生物成为长的和极化的成釉细胞样细胞的能力。我们的研究表明，颈环/牙周祖细胞在富含生长因子的培养液中共培养可形成高水平表达成釉蛋白和成釉蛋白的成釉细胞样细胞。将宫颈环状细胞与牙髓细胞共培养在含有富含亮氨酸的成釉蛋白多肽(LRAP)和早期釉质基质的量身定制的胶原支架上，可形成单一的、拉长的、极化的成釉细胞样细胞，表达和分泌成釉蛋白和成釉蛋白。富含釉质基质和LRAP的生物反应器微环境也被证明有利于HAT-7细胞的生长，导致成釉蛋白和成釉蛋白的∼表达比在普通支架上生长的对照组高20倍。总之，这里介绍的研究强调了微重力培养系统结合成釉细胞特有的微环境和定制的支架对成釉细胞样细胞生长的好处。
The growth of long and polarized ameloblast-like cells has long been heralded as a major prerequisite for enamel tissue engineering. In this study, we have designed three-dimensional bioreactor/scaffold microenvironments to propagate and assess the ability of cervical loop derivatives to become long and polarized ameloblast-like cells. Our studies demonstrated that cervical loop/periodontal progenitor coculture in a growth-factor-enriched medium resulted in the formation of ameloblast-like cells expressing high levels of amelogenin and ameloblastin. Coculture of cervical loop cells with dental pulp cells on tailored collagen scaffolds enriched with leucine-rich amelogenin peptide (LRAP) and early enamel matrix resulted in singular, elongated, and polarized ameloblast-like cells that expressed and secreted ameloblastin and amelogenin enamel proteins. Bioreactor microenvironments enriched with enamel matrix and LRAP also proved advantageous for the propagation of HAT-7 cells, resulting in a ∼20-fold higher expression of amelogenin and ameloblastin enamel proteins compared with controls growing on plain scaffolds. Together, studies presented here highlight the benefits of microgravity culture systems combined with ameloblast-specific microenvironments and tailored scaffolds for the growth of ameloblast-like cells.