A novel signaling molecule for neuropeptide action: Activity-dependent neuroprotective protein
A novel signaling molecule for neuropeptide action: Activity-dependent neuroprotective protein
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DOI：
10.1111/j.1749-6632.1999.tb07884.x
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发表时间：
1999-01-01
期刊：
影响因子：
--
通讯作者：
Brenneman, DE
中科院分区：
文献类型：
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作者：
Gozes, I;Bassan, M;Brenneman, DE
最近发现了一种新的蛋白质（828个氨基酸，pi 5，99）的完整编码序列，该蛋白质是一种潜在的血管活性肠肽（VIP）活性的新介质。活性依赖性神经保护蛋白（activity-dependent neuroprotective protein，ADNP）在VIP存在下，在大脑皮质星形胶质细胞中的表达增强，编码ADNP的mRNA转录产物仅在小鼠海马和小脑中富集。该蛋白质序列包含：（1）NAPVSIPQ，与已报道的活性依赖性神经营养因子（ADNF）具有结构和免疫学上的同源性，在体内外均具有神经保护作用;和（3）经典的锌结合结构域。比较研究表明，肽NAPVSIPQ（NAP）比衍生自ADNF，ADNP，VIP相关神经元存活的潜在介体，以及新的肽，用于药物设计的潜在先导化合物，将在下面讨论。
The complete coding sequence of a novel protein (828 amino acids, pi 5,99), a potential new mediator of vasoactive intestinal peptide (VIP) activity was recently revealed. The expression of this molecule, activity-dependent neuroprotective protein (ADNP), was augmented in the presence of VIP, in cerebral cortical astrocytes, The mRNA transcripts encoding ADNP mere enriched in the mouse hippocampus and cerebellum. The protein deduced sequence contained the following: (1) a unique peptide, NAPVSIPQ, sharing structural and immunological homologies with the previously reported, activity-dependent neurotrophic factor (ADNF) and exhibiting neuroprotection in vitro and in vivo; (2) a glutaredoxin active site; and (3) a classical zinc binding domain, Comparative studies suggested that the peptide, NAPVSIPQ (NAP), was more efficacious than peptides derived from ADNF, ADNP, a potential mediator of VIP-associated neuronal survival, and the new peptide, a potential lead compound for drug design, are discussed below.