Multigene family for sarcomeric myosin heavy chain in mouse and human DNA: localization on a single chromosome.
Multigene family for sarcomeric myosin heavy chain in mouse and human DNA: localization on a single chromosome.
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DOI：
10.1126/science.6879174
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发表时间：
1983-08
期刊：
影响因子：
56.9
通讯作者：
L. Leinwand;R. Fournier;B. Nadal-Ginard;T. Shows
中科院分区：
文献类型：
--
作者：
L. Leinwand;R. Fournier;B. Nadal-Ginard;T. Shows
克隆的大鼠和人肌球蛋白重链DNA探针与体细胞杂种及其亲本细胞基因组DNA的限制性内切酶酶切片段杂交。通过该测定可检测的小鼠肌球蛋白重链基因位于11号染色体上，并且三种不同的人肌节肌球蛋白重链基因被定位于17号染色体的短臂。肌球蛋白重链和两个不相关的标记，胸苷激酶和半乳糖激酶之间的同线性，被发现保存在啮齿动物和人类的基因组。
Cloned myosin heavy chain DNA probes from rat and human were hybridized to restriction endonuclease digests of genomic DNA from somatic cell hybrids and their parental cells. The mouse myosin heavy chain genes detectable by this assay were located on chromosome 11, and three different human sarcomeric myosin heavy chain genes were mapped to the short arm of chromosome 17. A synteny between myosin heavy chain and two unrelated markers, thymidine kinase and galactokinase, was found to be preserved in the rodent and human genomes.