An eight-parent multiparent advanced generation inter-cross population for winter-sown wheat: creation, properties, and validation.
An eight-parent multiparent advanced generation inter-cross population for winter-sown wheat: creation, properties, and validation.
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DOI：
10.1534/g3.114.012963
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发表时间：
2014-09-18
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影响因子：
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通讯作者：
Howell PJ
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作者：
Mackay IJ;Bansept-Basler P;Barber T;Bentley AR;Cockram J;Gosman N;Greenland AJ;Horsnell R;Howells R;O'Sullivan DM;Rose GA;Howell PJ
MAGIC群体代表了新一代作物遗传作图资源之一，结合了高遗传重组和多样性。我们描述了创建和验证的八个父母MAGIC人口组成的1091 F7系的冬播小麦（小麦L.）。基于90，000个单核苷酸多态性（SNP）阵列的基因型分析发现，该群体非常适合作为精细定位数量性状基因座（QTL）和基因分离的平台。连锁不平衡（LD）的模式显示人口高度重组的创始人之间的遗传标记多样性是74%，在一个更大的一组64个小麦品种，54%的SNPs分离的64行之间也分离的8个创始人行。相比之下，一个常用的参考双亲群体只有54%的64个品种的多样性与27%的SNPs分离。我们证明了这种MAGIC资源的潜力，通过确定一个高度诊断标记的形态特征“芒的存在/不存在”，并独立验证它的关联映射面板。这些分析表明，这个庞大、多样和高度重组的MAGIC群体是小麦目标性状遗传解剖的强大资源，并且能够有效地利用表型组学和基因组学的持续进展。遗传标记和性状数据以及获取种子的说明可在http://www.niab.com/MAGIC/上查阅。
MAGIC populations represent one of a new generation of crop genetic mapping resources combining high genetic recombination and diversity. We describe the creation and validation of an eight-parent MAGIC population consisting of 1091 F7 lines of winter-sown wheat (Triticum aestivum L.). Analyses based on genotypes from a 90,000-single nucleotide polymorphism (SNP) array find the population to be well-suited as a platform for fine-mapping quantitative trait loci (QTL) and gene isolation. Patterns of linkage disequilibrium (LD) show the population to be highly recombined; genetic marker diversity among the founders was 74% of that captured in a larger set of 64 wheat varieties, and 54% of SNPs segregating among the 64 lines also segregated among the eight founder lines. In contrast, a commonly used reference bi-parental population had only 54% of the diversity of the 64 varieties with 27% of SNPs segregating. We demonstrate the potential of this MAGIC resource by identifying a highly diagnostic marker for the morphological character "awn presence/absence" and independently validate it in an association-mapping panel. These analyses show this large, diverse, and highly recombined MAGIC population to be a powerful resource for the genetic dissection of target traits in wheat, and it is well-placed to efficiently exploit ongoing advances in phenomics and genomics. Genetic marker and trait data, together with instructions for access to seed, are available at http://www.niab.com/MAGIC/.