Genome sequencing of mouse induced pluripotent stem cells reveals retroelement stability and infrequent DNA rearrangement during reprogramming.
Genome sequencing of mouse induced pluripotent stem cells reveals retroelement stability and infrequent DNA rearrangement during reprogramming.
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DOI：
10.1016/j.stem.2011.07.018
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发表时间：
2011-10-04
期刊：
影响因子：
23.9
通讯作者：
Hall, Ira M.
中科院分区：
文献类型：
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作者：
Quinlan, Aaron R.;Boland, Michael J.;Leibowitz, Mitchell L.;Shumilina, Svetlana;Pehrson, Sidney M.;Baldwin, Kristin K.;Hall, Ira M.
The biomedical utility of induced pluripotent stem cells (iPSCs) will be diminished if most iPSC lines harbor deleterious genetic mutations. Recent microarray studies have shown that human iPSCs carry elevated levels of DNA copy number variation compared to embryonic stem cells, suggesting that these and other classes of genomic structural variation (SV) including inversions, smaller duplications and deletions, complex rearrangements and retroelement transpositions may frequently arise as a consequence of reprogramming. Here we employ whole genome paired-end DNA sequencing and sensitive mapping algorithms to identify all classes of SV in several fully pluripotent mouse iPSC lines. Despite the improved scope and resolution of this study, we find few spontaneous mutations per line (1–2) and no evidence for endogenous retroelement transposition. These results show that genome stability can persist throughout reprogramming, and argue that it is possible to generate iPSCs lacking gene disrupting mutations using current reprogramming methods.
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DOI：
10.1093/bioinformatics/btq033
发表时间：
2010-03-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
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作者：
Quinlan AR;Hall IM
通讯作者：
Hall IM
重编程因子表达引发了广泛的靶向染色质重塑。
影响因子：
23.9
作者：
Koche RP;Smith ZD;Adli M;Gu H;Ku M;Gnirke A;Bernstein BE;Meissner A
通讯作者：
Meissner A
影响因子：
64.8
作者：
Muotri, AR;Chu, VT;Gage, FH
通讯作者：
Gage, FH
影响因子：
11.4
作者：
Deng, Wenbin;Xu, Yang
通讯作者：
Xu, Yang
影响因子：
64.8
作者：
通讯作者：
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