Lipid raft microdomains: a gateway for compartmentalized trafficking of Ebola and Marburg viruses.
Lipid raft microdomains: a gateway for compartmentalized trafficking of Ebola and Marburg viruses.
复制标题
DOI：
10.1084/jem.20011500
复制
发表时间：
2002-03-04
期刊：
影响因子：
--
通讯作者：
Aman MJ
中科院分区：
文献类型：
--
作者：
Bavari S;Bosio CM;Wiegand E;Ruthel G;Will AB;Geisbert TW;Hevey M;Schmaljohn C;Schmaljohn A;Aman MJ
丝状病毒进入和释放的时空方面知之甚少。脂筏作为多种细胞信号和运输过程的功能平台。在这里，我们报告了埃博拉和马尔堡病毒蛋白在病毒组装和萌发过程中在脂筏中的区隔。从受感染细胞释放的丝状病毒结合了RAFT相关分子，表明病毒在筏上退出。异位表达的埃博拉病毒基质蛋白和糖蛋白支持RAFT依赖的丝状、病毒样颗粒(VLP)的释放，电子显微镜显示这种释放与活病毒非常相似。我们的发现还显示，丝状病毒的进入需要有功能的木筏，确定木筏是病毒攻击的地点。将RAFT确定为丝状病毒进出的通道和依赖RAFT产生的VLP对于开发针对埃博拉和马尔堡病毒感染的治疗和疫苗策略具有重要意义。
Spatiotemporal aspects of filovirus entry and release are poorly understood. Lipid rafts act as functional platforms for multiple cellular signaling and trafficking processes. Here, we report the compartmentalization of Ebola and Marburg viral proteins within lipid rafts during viral assembly and budding. Filoviruses released from infected cells incorporated raft-associated molecules, suggesting that viral exit occurs at the rafts. Ectopic expression of Ebola matrix protein and glycoprotein supported raft-dependent release of filamentous, virus-like particles (VLPs), strikingly similar to live virus as revealed by electron microscopy. Our findings also revealed that the entry of filoviruses requires functional rafts, identifying rafts as the site of virus attack. The identification of rafts as the gateway for the entry and exit of filoviruses and raft-dependent generation of VLPs have important implications for development of therapeutics and vaccination strategies against infections with Ebola and Marburg viruses.
登录
查看更多内容
影响因子：
64.5
作者：
Dimitrov, DS
通讯作者：
Dimitrov, DS
埃博拉病毒基质蛋白 VP40 的晶体结构
影响因子：
11.4
作者：
Dessen, A;Volchkov, V;Weissenhorn, W
通讯作者：
Weissenhorn, W
影响因子：
56.9
作者：
Gatfield, J;Pieters, J
通讯作者：
Pieters, J
影响因子：
7.8
作者：
Harder, T;Scheiffele, P;Verkade, P;Simons, K
通讯作者：
Simons, K
影响因子：
5
作者：
Geisbert, TW;Jahrling, PB
通讯作者：
Jahrling, PB