GENES HOMOLOGOUS TO UBIQUITIN-CONJUGATING PROTEINS AND EUKARYOTIC TRANSCRIPTION FACTOR-SII IN AFRICAN SWINE FEVER VIRUS
GENES HOMOLOGOUS TO UBIQUITIN-CONJUGATING PROTEINS AND EUKARYOTIC TRANSCRIPTION FACTOR-SII IN AFRICAN SWINE FEVER VIRUS
复制标题
DOI：
10.1016/0042-6822(92)90059-x
复制
发表时间：
1992-01-01
期刊：
影响因子：
3.7
通讯作者：
VINUELA, E
中科院分区：
文献类型：
--
作者：
RODRIGUEZ, JM;SALAS, ML;VINUELA, E
已经确定了非洲猪瘟病毒DNA 6004 bp EcoRl I片段的核苷酸序列。该序列的翻译发现了8个紧密间隔的开放阅读框架（orf），其中3个向右阅读，5个向左阅读。Northern blot杂交分析表明，orfs173r和178R在感染早期表达，而ORFsI177L、I196L和I329L在感染后期表达。ORFs I215L、I226R和I243L转录本在早期RNA中含量较低，在晚期RNA中含量较高。基因I73R/I329L和基因I7811/I215L之间的基因间区存在直接重复序列。在orf1196l中也发现了直接重复。ORF I329L编码的蛋白含有一个假定可切割的信号肽和一个内部跨膜结构域，ORF I177L编码的蛋白具有一个具有“启停”序列特征的氨基端疏水区。ORF I243L编码一种类似于真核延伸因子SII的蛋白。ORF 1215L编码的蛋白与泛素结合蛋白家族同源。
The nucleotide sequence of the 6004-bp EcoRl I fragment of African swine fever virus DNA has been determined. Translation of the sequence revealed eight closely spaced open reading frames (ORFs), three of them reading rightward and five leftward. Northern blot hybridization analysis indicated that ORFs 173R and 178R were transcribed early in infection, whereas ORFsI177L, I196L, and I329L were expressed at late times. Transcripts for ORFs I215L, I226R, and I243L were detected at low levels in early RNA and at higher levels in late RNA. The intergenic regions between genes I73R/I329L and I7811/I215L were characterized by the presence of direct repeats in tandem. Direct repetitions were also found within ORF 1196L. The protein encoded by ORF I329L contained a putative cleavable signal peptide and an internal transmembrane domain, and that encoded by ORF I177L had an amino-terminal hydrophobic region with the characteristics of a “start-stop” sequence. ORF I243L encoded a protein similar to the eukaryotic elongation factor SII. The protein encoded by ORF 1215L was homologous to the family of ubiquitin-conjugating proteins.