Light-induced retinal changes observed with high-resolution autofluorescence imaging of the retinal pigment epithelium
Light-induced retinal changes observed with high-resolution autofluorescence imaging of the retinal pigment epithelium
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DOI：
10.1167/iovs.07-1430
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发表时间：
2008-08-01
影响因子：
4.4
通讯作者：
Williams, David R.
中科院分区：
文献类型：
--
作者：
Morgan, Jessica I. W.;Hunter, Jennifer J.;Williams, David R.
目的。使用自适应光学扫描激光眼底镜(AOSLO)的自体荧光眼底成像允许在体内对单个视网膜色素上皮(RPE)细胞进行成像。在这项研究中，通过使用2到150倍于常规成像的辐射暴露水平来研究视网膜损伤的可能性。方法：用荧光AOSLO对猕猴视网膜进行活体成像。视网膜被暴露在568或830 nm的光中，以不同的强度在每边1/2度的范围内照射15分钟。在曝光前和曝光后立即拍摄感光细胞和RPE细胞的2个视野的图像。照射后5~165d间歇性地进行AOSLO长期显像。结果：暴露于568 nm的光可立即降低RPE细胞的自发荧光。随访成像显示，暴露时RPE细胞的自体荧光完全恢复或长期受损。平均功率相等的AOSLO暴露和均匀野暴露的结果没有显著差异。830 nm的照射没有观察到任何影响。结论：本研究揭示了568 nm照射引起的RPE自体荧光的新变化。视网膜损伤的发生是总平均功率的直接结果，与光传输方法无关。由于暴露量接近或低于激光安全标准中允许的水平，这些结果表明，对视网膜暴露在可见光下应谨慎行事，并应重新评估这些暴露条件下的安全标准。
PURPOSE. Autofluorescence fundus imaging using an adaptive optics scanning laser ophthalmoscope (AOSLO) allows for imaging of individual retinal pigment epithelial (RPE) cells in vivo. In this study, the potential of retinal damage was investigated by using radiant exposure levels that are 2 to 150 times those used for routine imaging.METHODS. Macaque retinas were imaged in vivo with a fluorescence AOSLO. The retina was exposed to 568-or 830-nm light for 15 minutes at various intensities over a square 1/2 degrees per side. Pre- and immediate postexposure images of the photoreceptors and RPE cells were taken over a 2 field. Long-term AOSLO imaging was performed intermittently from 5 to 165 days after exposure. Exposures delivered over a uniform field were also investigated.RESULTS. Exposures to 568-nm light caused an immediate decrease in autofluorescence of RPE cells. Follow-up imaging revealed either full recovery of autofluorescence or long-term damage in the RPE cells at the exposure. The outcomes of AOSLO exposures and uniform field exposures of equal average power were not significantly different. No effects from 830-nm exposures were observed.CONCLUSIONS. The study revealed a novel change in RPE autofluorescence induced by 568-nm light exposure. Retinal damage occurred as a direct result of total average power, independent of the light-delivery method. Because the exposures were near or below permissible levels in laser safety standards, these results suggest that caution should be used with exposure of the retina to visible light and that the safety standards should be re-evaluated for these exposure conditions.