Continuous measurement of protein osmotic pressure in blood and lymph of sheep.
Continuous measurement of protein osmotic pressure in blood and lymph of sheep.
复制标题
连续测量绵羊血液和淋巴液中的蛋白质渗透压。
DOI：
10.1152/jappl.1988.64.2.869
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Staub,NC
中科院分区：
文献类型：
--
作者：
Yamada,S;Grady,M;Staub,NC
我们连续测定了绵羊血液和淋巴液的蛋白质渗透压，比较了两种针式渗透压计（刚性和柔性）和膜式渗透压计（Wescor）。我们还比较了连续测量的平均值与使用Yamada方程从总蛋白和白蛋白组分计算的渗透压。刚性针渗透压计和膜渗透压计显示出良好的相关性（y = 1.00x + 0.06; r> 0.99）。软针渗透压计倾向于高估渗透压（平均16%）。采用硬针渗透压计连续测定麻醉和非麻醉绵羊血液和淋巴蛋白质渗透压，观察三种不同干预措施对血液和淋巴蛋白质渗透压的影响。渗透压的理论值（x）与连续测量值（y）之间的关系良好（y = 0.99x + 0.16，r = 0.97），但在各种干预后，连续测量的蛋白质渗透压有超过计算值的趋势。应使用固定式渗透压计中测量的点样或通过总蛋白浓度和白蛋白分数计算渗透压来监测连续测量。
We have continuously measured protein osmotic pressure of blood and lymph in sheep to compare two kinds of needle osmometers (rigid and flexible) with a membrane osmometer (Wescor). We also compared the averaged values of the continuous measurement with osmotic pressure calculated from total protein and albumin fraction, using the Yamada equation. The rigid-needle and membrane osmometers showed excellent correlation (y = 1.00x + 0.06; r greater than 0.99). The flexible-needle osmometer tended to overestimate osmotic pressure (avg 16%). We used the rigid-needle osmometer for continuous measurements of protein osmotic pressure of blood and lymph in anesthetized or unanesthetized sheep to observe changes in protein osmotic pressure of blood and lymph through the three different interventions. The relationship between the theoretical values (x) and the continuous measurements (y) of osmotic pressure was good (y = 0.99x + 0.16, r = 0.97), but after various interventions, the continuously measured protein osmotic pressure tended to exceed the calculated measurements. The continuous measurement should be monitored with spot samples measured in a stationary osmometer or by calculation of osmotic pressure from total protein concentration and albumin fraction.