Arsenic methylation capability, heme oxygenase-1 and NADPH quinone oxidoreductase-1 genetic Polymorphisms and the stage and grade of urothelial carcinomas
Arsenic methylation capability, heme oxygenase-1 and NADPH quinone oxidoreductase-1 genetic Polymorphisms and the stage and grade of urothelial carcinomas
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DOI：
10.1159/000132699
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发表时间：
2008-01-01
影响因子：
1.6
通讯作者：
Hsueh, Yu-Mei
中科院分区：
文献类型：
--
作者：
Huang, Steven K.;Chiu, Allen Wen-Hsiang;Hsueh, Yu-Mei
背景：砷暴露与尿路上皮癌（UC）风险增加相关。为探讨砷甲基化能力在不同分期和分级UC患者中的分布，本研究于2002年9月至2004年5月期间招募了100例UC患者。研究方法：采用高效液相色谱-氢化物发生-原子吸收光谱法测定尿中无机砷（As（III）+ As-V）、甲基胂酸（MMA）和二甲基胂酸（DMA）。测定各种砷形态占尿砷总量的百分比，评估砷甲基化能力。初级甲基化指数（PMI）定义为MMA和无机砷之间的比值。二级甲基化指数（SMI）被确定为DMA和MMA之间的比率。结果如下：砷甲基化能力在不同UC阶段的患者中存在差异性影响;但在不同级别之间没有发现任何影响。结论：HO-1基因型在不同阶段UC患者中的分布有显著差异，但与NAD（P）H：醌氧化还原酶1基因型无关。版权所有（c）2008 S. Karger AG，巴塞尔。
Background: Arsenic exposure is associated with an increased risk of urothelial carcinoma ( UC). To explore the distribution of the arsenic methylation capability in patients with different stages and grades of UCs, 100 UC cases were recruited between September 2002 and May 2004 for this study. Methods: Urinary arsenic species, including inorganic arsenic (As (III) + As-V), monomethylarsonic acid ( MMA), and dimethylarsinic acid ( DMA), were determined with a high- performance liquid chromatography- linked hydride generator and atomic absorption spectrometry. Determining the percentages of various arsenic species among the total urinary arsenic amount assessed the arsenic methylation capability. The primary methylation index ( PMI) was defined as the ratio between MMA and inorganic arsenic. The secondary methylation index ( SMI) was determined as the ratio between DMA and MMA. Results: Differential effects of the arsenic methylation capability were found among patients with different stages of UCs; however, none was found among different grades. Conclusion: A significantly different distribution of the HO-1 genotype was found in subjects with different- stage UCs; however, it was not related to the NAD( P) H: quinone oxidoreductase 1 genotype. Copyright (c) 2008 S. Karger AG, Basel.