The impact of different nanoparticle surface chemistry and size on uptake and toxicity in a murine macrophage cell line
The impact of different nanoparticle surface chemistry and size on uptake and toxicity in a murine macrophage cell line
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DOI：
10.1016/j.taap.2008.06.009
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发表时间：
2008-11-01
影响因子：
3.8
通讯作者：
Stone, Vicki
中科院分区：
文献类型：
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作者：
Clift, Martin J. D.;Rothen-Rutishauser, Barbara;Stone, Vicki
本研究研究了不同表面涂层量子点（QD）的吸收、动力学和细胞分布，然后将其与其毒性联系起来。用有机物、COOH和NH 2（PEG）表面包被的QD（40 nM）处理J774.A1细胞。模型20 nm和200 nm COOH-改性包被的聚苯乙烯珠（PB）也进行了检查（50 μ g ml（-1））。通过固定和活细胞共聚焦显微镜以及流式细胞术在2小时内检查QD摄取的潜力。COOH 20 nm和200 nm PB均被J774.A1细胞明显且快速摄取，20 nm PB的摄取相对更快且更广泛。类似地，COOH QD明显被巨噬细胞摄取。NH 2（PEG）QD的摄取通过活细胞成像检测不到，然而，在固定细胞的3D重建后以及通过流式细胞术观察到。用有机量子点处理的细胞，通过活细胞成像监测，在相对少量的细胞中仅显示少量的摄取。这种摄取不足以通过流式细胞术检测。由于细胞毒性相关的细胞完整性损失，固定细胞无法成像。在无细胞环境中，在pH 4.0（指示内体）下2小时后，发现有机QD、NH 2（PEG）QD、20 nm和200 nm PB的荧光强度显著降低（p < 0.05），表明稳定性降低。在2小时内未发现胞吐的证据。这些发现证实了表面涂层对NP与细胞相互作用的模式以及该相互作用的后续后果具有显著影响。皇冠版权所有（C）2008由爱思唯尔公司出版。All rights reserved.
This study investigated the uptake, kinetics and cellular distribution of different surface coated quantum dots (QDs) before relating this to their toxicity. J774.A1 cells were treated with organic, COOH and NH2 (PEG) surface coated QDs (40 nM). Model 20 nm and 200 nm COOH-modified coated polystyrene beads (PBs) were also examined (50 mu g ml(-1)). The potential for uptake of QDs was examined by both fixed and live cell confocal microscopy as well as by flow cytometry over 2 h. Both the COOH 20 nm and 200 nm PBs were clearly and rapidly taken up by the J774.A1 cells, with uptake of 20 nm PBs being relatively quicker and more extensive. Similarly, COOH QDs were clearly taken up by the macrophages. Uptake of NH2 (PEG) QDs was not detectable by live cell imaging however, was observed following 3D reconstruction of fixed cells, as well as by flow cytometry. Cells treated with organic QDs, monitored by live cell imaging, showed only a small amount of uptake in a relatively small number of cells. This uptake was insufficient to be detected by flow cytometry. Imaging of fixed cells was not possible due to a loss in cell integrity related to cytotoxicity. A significant reduction (p < 0.05) in the fluorescent intensity in a cell-free environment was found with organic QDs, NH2 (PEG) QDs, 20 nm and 200 nm PBs at pH 4.0 (indicative of an endosome) after 2 h, suggesting reduced stability. No evidence of exocytosis was found over 2 h. These findings confirm that surface coating has a significant influence on the mode of NP interaction with cells, as well as the subsequent consequences of that interaction. Crown Copyright (C) 2008 Published by Elsevier Inc. All rights reserved.