A hyperimmune serum against a synthetic peptide corresponding to the hypervariable region 1 of hepatitis C virus can prevent viral infection in cell cultures.
A hyperimmune serum against a synthetic peptide corresponding to the hypervariable region 1 of hepatitis C virus can prevent viral infection in cell cultures.
复制标题
针对与丙型肝炎病毒高变区1相对应的合成肽的超免疫血清可以预防细胞培养物中的病毒感染。
DOI：
10.1006/viro.1996.0497
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发表时间：
1996
期刊：
影响因子：
3.7
通讯作者：
H. Yoshikura
中科院分区：
文献类型：
--
作者：
Y. Shimizu;H. Igarashi;T. Kiyohara;T. Cabezón;P. Farci;R. Purcell;H. Yoshikura
To investigate whether a principal neutralization epitope exists in hypervariable region 1 (HVR1) within the putative envelope of hepatitis C virus (HCV), we generated a hyperimmune rabbit serum against a synthetic peptide corresponding to HVR1 of HCV isolate H77. The reactivity of the serum in the enzyme-linked immunosorbent assay was correlated with the 13 amino acids (position 398-410) in HVR1. The serum prevented infection with H77 virus in cell cultures but did not prevent infection with H90 virus, a genetically divergent isolate from the same patient. The study demonstrated that neutralization of HCV was mediated, in part, by isolate-specific antibody recognizing HVR1.
