THE SCREW GENE ENCODES A UBIQUITOUSLY EXPRESSED MEMBER OF THE TGF-BETA FAMILY REQUIRED FOR SPECIFICATION OF DORSAL CELL FATES IN THE DROSOPHILA EMBRYO
THE SCREW GENE ENCODES A UBIQUITOUSLY EXPRESSED MEMBER OF THE TGF-BETA FAMILY REQUIRED FOR SPECIFICATION OF DORSAL CELL FATES IN THE DROSOPHILA EMBRYO
复制标题
DOI：
10.1101/gad.8.21.2588
复制
发表时间：
1994-11-01
影响因子：
10.5
通讯作者：
OCONNOR, MB
中科院分区：
文献类型：
--
作者：
ARORA, K;LEVINE, MS;OCONNOR, MB
十肢麻痹（dpp）基因产物，TGF-β相关的配体，作为细胞外形态形成建立至少两个细胞反应阈值内的果蝇胚胎背侧的一半。screw基因的突变在表型上类似于中度dpp突变体，并导致背侧细胞采用腹侧命运。我们发现，sew编码一种新的TGF-β蛋白，是指定背纹信号的一个组成部分。虽然SEW在胚盘阶段均匀表达，但其对发育的影响似乎是分级的，并且仅限于胚胎的背侧。我们的研究结果表明，DPP活动本身是不足以指定不同的背细胞的命运。我们建议，SCW和DPP一起行动，以建立不同的反应边界内的背侧一半的胚胎，也许是通过形成异源二聚体，具有更高的活性比同型二聚体的分子单独。
The decapentaplegic (dpp) gene product, a TGF-beta related ligand, acts as an extracellular morphogen to establish at least two cellular response thresholds within the dorsal half of the Drosophila embryo. Null mutations in the screw (scw) gene are phenotypically similar to moderate dpp mutants and cause dorsal cells to adopt ventral fates. We show that sew encodes a novel TGF-beta protein and is an integral part of the signal that specifies dorsal pattern. Although sew is expressed uniformly during blastoderm stages, its effect on development appears graded and is restricted to the dorsal side of the embryo. Our results indicate that DPP activity alone is insufficient to specify different dorsal cell fates. We propose that SCW and DPP act together to establish distinct response boundaries within the dorsal half of the embryo, perhaps by forming heterodimers that have higher activity than homodimers of either molecule alone.