STX2 drives colorectal cancer proliferation via upregulation of EXOSC4
STX2 drives colorectal cancer proliferation via upregulation of EXOSC4
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STX2 通过上调 EXOSC4 驱动结直肠癌增殖
DOI：
10.1016/j.lfs.2020.118597
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发表时间：
2020-12-15
期刊：
影响因子：
6.1
通讯作者：
He, Guo-Yang
中科院分区：
文献类型：
--
作者：
Wang, Yong-Xia;Li, Yong-Zhen;He, Guo-Yang
目的：探讨突触融合蛋白2（Syntaxin 2，STX 2）在结直肠癌（Colorectal Cancer，CRC）增殖中的生物学功能及其作用机制。主要方法：通过一系列的功能获得性和功能丧失性分析来探讨STX 2在体内外CRC增殖中的生物学功能。采用Western blot、免疫共沉淀（Co-IP）和功能分析等方法分析STX 2对EXOSC 4在结直肠癌增殖中的调控作用;采用免疫组化（IHC）和实时定量聚合酶链反应（qPCR）进一步验证STX 2和EXOSC 4在结直肠癌组织中表达的相关性。主要发现：STX 2过表达促进CRC增殖，STX 2敲低抑制CRC增殖; STX 2通过增加EXOSC 4蛋白表达促进CRC增殖; STX 2与EXOSC 4表达呈正相关。意义：目前的数据证实STX 2通过增加EXOSC 4的表达来驱动CRC的增殖。
Aims: To explore the biological function and mechanism of Syntaxin2 (STX2) in Colorectal cancer (CRC) proliferation. Main methods: A series of gainand loss-of-function analysis were conducted the to explore the biological function of STX2 in CRC proliferation in vivo and in vitro. Western blot, Co-immunoprecipitation (Co-IP) and the functional analyses were taken to analyze the regulative role of STX2 on Exosome Complex 4 (EXOSC4) in CRC proliferation; Immunohistochemistry (IHC) and Real-time quantitative polymerase chain reaction (qPCR) were used to further verify the relationship between the expression of STX2 and EXOSC4 in human CRC samples. Key findings: Our study revealed that the over-expression of STX2 promoted CRC proliferation, while knockdown of STX2 repressed CRC proliferation; STX2 promoted CRC proliferation via increasing EXOSC4 protein; There was a positive correlation between STX2 and EXOSC4 expression. Significance: The current data verify that STX2 drives the proliferation of CRC via increasing the expression of EXOSC4.