The suitability of different cellular in vitro immunotoxicity and genotoxicity methods for the analysis of nanoparticle-induced events
The suitability of different cellular in vitro immunotoxicity and genotoxicity methods for the analysis of nanoparticle-induced events
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DOI：
10.3109/17435390903374001
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发表时间：
2010-03-01
期刊：
影响因子：
5
通讯作者：
Oostingh, Gertie J.
中科院分区：
文献类型：
--
作者：
Pfaller, Tobias;Colognato, Renato;Oostingh, Gertie J.
需要适当的检测方法和检测策略来分析纳米颗粒暴露可能造成的遗传毒性和免疫毒性健康影响。这种方法的开发和验证是具有挑战性的，因为当进行常规的体外分析时，纳米颗粒可能会表现出意想不到的行为，比如聚集或干扰光学测量。在我们的跨学科研究中，使用不同的检测系统测试了尺寸分布较窄的无机金(4.5 nm)和氧化铁(7.3 nm)纳米颗粒对人细胞的影响。结果表明，这两种无机纳米颗粒的细胞毒性、免疫毒性和遗传毒性在不同实验室的一组细胞测试中都很低或根本不存在。然而，必须解决几个特定于纳米颗粒工作的技术问题。本文对所使用的方法、它们在纳米毒性测试中的适用性以及遇到的技术问题进行了详细的描述和讨论。
Suitable assays and test strategies are needed to analyze potential genotoxic and immunotoxic health effects caused by nanoparticle exposure. The development and validation of such methods is challenging because nanoparticles may show unexpected behavior, like aggregation or interference with optical measurements, when routine in vitro assays are performed. In our interdisciplinary study, the effects of inorganic gold (4.5 nm) and iron oxide (7.3 nm) nanoparticles with a narrow size distribution were tested on human cells using different assay systems. The results show that cytotoxicity as well as immunotoxicity and genotoxicity induced by these two inorganic nanoparticles was low or absent when using a panel of cell-based tests in different laboratories. However, several technical issues had to be tackled that were specific for working with nanoparticles. The methods used, their suitability for nanotoxicity testing, and the technical problems encountered are carefully described and discussed in this paper.