Genome-wide identification and expression analysis of the ClTCP transcription factors in Citrullus lanatus.
Genome-wide identification and expression analysis of the ClTCP transcription factors in Citrullus lanatus.
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西瓜 ClTCP 转录因子的全基因组鉴定及表达分析
DOI：
10.1186/s12870-016-0765-9
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发表时间：
2016-04-12
影响因子：
5.3
通讯作者：
Hu Z
中科院分区：
文献类型：
--
作者：
Shi P;Guy KM;Wu W;Fang B;Yang J;Zhang M;Hu Z
背景：植物特异性TCP转录因子家族参与细胞生长和增殖的调控，在植物生长发育的多个方面发挥着不同的功能。然而，没有全面的分析西瓜（西瓜）TCP家族已进行previous.Results：共27西瓜TCP编码基因分布在9条染色体上。系统发育分析将这些基因分为11个不同的亚组。此外，系统发育和结构分析区分ClTCP家族内的两个同源类，指定为I类和II类。II类基因分为两个亚类，CIN亚类和CYC/TB 1亚类。通过半定量PCR确定所有成员的表达模式。两个ClTCP基因，ClTCP 14 a和ClTCP 15，在拟南芥野生型和直系同源突变体中的异位表达证实了在调节株高的功能。结论：这项研究代表了西瓜TCP基因家族的第一个全基因组分析，这为了解西瓜TCP基因的分类和功能提供了有价值的信息。
Background:The plant-specific TCP transcription factor family, which is involved in the regulation of cell growth and proliferation, performs diverse functions in multiple aspects of plant growth and development. However, no comprehensive analysis of the TCP family in watermelon (Citrullus lanatus) has been undertaken previously.Results:A total of 27 watermelon TCP encoding genes distributed on nine chromosomes were identified. Phylogenetic analysis clustered the genes into 11 distinct subgroups. Furthermore, phylogenetic and structural analyses distinguished two homology classes within the ClTCP family, designated Class I and Class II. The Class II genes were differentiated into two subclasses, the CIN subclass and the CYC/TB1 subclass. The expression patterns of all members were determined by semi-quantitative PCR. The functions of two ClTCP genes, ClTCP14a and ClTCP15, in regulating plant height were confirmed by ectopic expression in Arabidopsis wild-type and ortholog mutants.Conclusions:This study represents the first genome-wide analysis of the watermelon TCP gene family, which provides valuable information for understanding the classification and functions of the TCP genes in watermelon.