Autographa Californica Multiple Nucleopolyhedrovirus P48 (Ac103) Is Required for the Efficient Formation of Virus-Induced Intranuclear Microvesicles
Autographa Californica Multiple Nucleopolyhedrovirus P48 (Ac103) Is Required for the Efficient Formation of Virus-Induced Intranuclear Microvesicles
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DOI：
10.1007/s12250-019-00147-8
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发表时间：
2019-07
影响因子：
5.5
通讯作者：
Yan Wang;Qing-mei Cai;Jiannan Chen;Zhihong Huang;Wenbi Wu;M. Yuan;Kai Yang
中科院分区：
文献类型：
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作者：
Yan Wang;Qing-mei Cai;Jiannan Chen;Zhihong Huang;Wenbi Wu;M. Yuan;Kai Yang
我们先前的研究表明，加州拟珠藻多核型多角体病毒(AcMNPV)P48(Ac103)基因对于核衣壳的核外泄和包涵体衍生病毒粒子(ODV)的形成是必不可少的。然而，p48在ODV形态发生中的确切作用仍不清楚。在这项研究中，我们证明了p48是有效形成核内微泡所必需的。为了进一步了解它在核内微泡形成中的功能作用，我们研究了P48蛋白的分布，发现它与萌发的病毒粒子和ODV的核衣壳和包膜部分有关。在AcMNPV感染的细胞中，P48主要定位于病毒基质中的核衣壳和ODV包膜中的核衣壳，在质膜、核膜、核内微泡和ODV包膜中有少量但可辨别的分布。此外，免疫共沉淀分析表明，在核内微泡形成所需的病毒蛋白中，P48与Ac93在没有病毒感染的情况下相关。
Our previous study has shown that the Autographa californica multiple nucleopolyhedrovirus (AcMNPV)p48(ac103) gene is essential for the nuclear egress of nucleocapsids and the formation of occlusion-derived virions (ODVs). However, the exact role ofp48in the morphogenesis of ODVs remains unknown. In this study, we demonstrated thatp48was required for the efficient formation of intranuclear microvesicles. To further understand its functional role in intranuclear microvesicle formation, we characterized the distribution of the P48 protein, which was found to be associated with the nucleocapsid and envelope fractions of both budded virions and ODVs. In AcMNPV-infected cells, P48 was predominantly localized to nucleocapsids in the virogenic stroma and the nucleocapsids enveloped in ODVs, with a limited but discernible distribution in the plasma membrane, nuclear envelope, intranuclear microvesicles, and ODV envelope. Furthermore, coimmunoprecipitation assays showed that among the viral proteins required for intranuclear microvesicle formation, P48 associated with Ac93 in the absence of viral infection.