LACK OF DETECTION OF ENTEROVIRAL RNA OR BACTERIAL-DNA IN MAGNETIC-RESONANCE IMAGING-DIRECTED MUSCLE BIOPSIES FROM 20 CHILDREN WITH ACTIVE UNTREATED JUVENILE DERMATOMYOSITIS
LACK OF DETECTION OF ENTEROVIRAL RNA OR BACTERIAL-DNA IN MAGNETIC-RESONANCE IMAGING-DIRECTED MUSCLE BIOPSIES FROM 20 CHILDREN WITH ACTIVE UNTREATED JUVENILE DERMATOMYOSITIS
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DOI：
10.1002/art.1780381019
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发表时间：
1995-10-01
影响因子：
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通讯作者：
PALLANSCH, M
中科院分区：
文献类型：
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作者：
PACHMAN, LM;LITT, DL;PALLANSCH, M
目的，调查活动性新发青少年皮肌炎 (DM) 儿童中是否存在针对假定感染原的循环抗体滴度增加以及可检测到的病毒 RNA 或细菌 DNA。方法，对 20 名活动性、未经治疗的新发青少年 DM 儿童和年龄匹配的神经系统疾病儿童进行了磁共振成像引导的肌肉活检， 检测血清中针对柯萨奇病毒 B (CVB)、甲型和乙型流感病毒、副流感 1 和 3 型、肺炎支原体、腮腺炎、呼吸道合胞病毒和呼肠孤病毒的补体固定抗体；并通过免疫荧光法检测弓形虫巨细胞病毒的 IgG 抗体和 Epstein-Barr 病毒的 IgM 抗体，使用 5 个引物组的逆转录酶聚合酶链反应测试来自青少年 DM 患者和对照儿童的肌肉、感染和未感染 CVB1 的 CD-1 Swiss 小鼠以及 CVB1-6 阳性病毒储液，4 探针（1个柯萨奇病毒，3个肠道病毒）和DNA通用引物，结果，青少年DM患者或对照儿童中不存在抗体、病毒RNA或细菌DNA增加，结论。青少年糖尿病可能是由遗传易感宿主体内的不明物质引发的。
Objective, To investigate for the presence of increased titers of circulating antibody to putative infectious agents and for detectable viral RNA or bacterial DNA in children with active recent-onset juvenile dermatomyositis (DM),Methods, Magnetic resonance imaging-directed muscle biopsies were performed in 20 children with active, untreated, recent-onset juvenile DM and in age-matched children with neurologic disease, Sera were tested for complement-fixing antibody to Coxsackievirus B (CVB), influenza A and B, parainfluenza 1 and 3, Mycoplasma pneumoniae, mumps, respiratory syncytial virus, and Reovirus; and by immunofluorescence for IgG antibody to Toxoplasma gondii cytomegalovirus and IgM antibody to Epstein-Barr virus, Muscle from juvenile DM patients and control children, CD-1 Swiss mice with and without CVB1 infection, and viral stock positive for CVB1-6 were tested using reverse-transcriptase polymerase chain reaction with 5 primer sets, 4 probes (1 Coxsackievirus, 3 Enterovirus), and universal primers for DNA,Results, No increased antibody, viral RNA, or bacterial DNA was present in the juvenile DM patients or the control children,Conclusion. Juvenile DM may be triggered by unidentified agent(s) in the genetically susceptible host.