Genome-wide analysis of RNA-protein interactions illustrates specificity of the mRNA export machinery
Genome-wide analysis of RNA-protein interactions illustrates specificity of the mRNA export machinery
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DOI：
10.1038/ng1080
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发表时间：
2003-02-01
期刊：
影响因子：
30.8
通讯作者：
Silver, PA
中科院分区：
文献类型：
--
作者：
Hieronymus, H;Silver, PA
MRNA的核输出是由一种复杂的RNA结合蛋白机制介导的，该机制识别并通过信使核糖核蛋白(MRNP)网络传递mRNAs。任何特定的信使核糖核酸输出因子的全谱信使核糖核酸都是未知的。我们鉴定了与两个保守的酵母mRNA输出因子Yra1(参考文献1)结合的mRNAs。1-5)和Mex67(参考文献6，7)，并确定了它们的结合水平。Yra1和Mex67分别结合了大约1,000和1,150个mRNAs，分别相当于酵母基因组的近20%和所有转录事件的大约36%。Yra1靶标的结合水平与其转录频率有关，而Mex67靶标的结合水平与其转录频率无关。Yra1结合的转录本富含受许多转录因子调控的mRNAs。Yra1和Mex67结合的群体也显示出编码不同功能类蛋白质的mRNAs的丰富，其中一些受到这些转录因子的调节。我们确定了一个这样的转录因子，Abf1(参考文献)。8-10)，与Yra1关联。这些结果表明，mRNA输出因子具有以前未知的特异性，它可能通过与转录机制的相互作用来协调转录共同调节的功能类转录产物的输出。
Nuclear export of mRNA is mediated by a complex machinery of RNA-binding proteins that recognizes and routes mRNAs through a messenger ribonucleoprotein (mRNP) network. The full spectrum of mRNA cargoes for any dedicated mRNA export factor is unknown. We identified the mRNAs that bind two conserved yeast mRNA export factors, Yra1 (refs. 1-5) and Mex67 (refs. 6,7), on a genome-wide scale and determined their level of binding. Yra1 and Mex67 bind approximately 1,000 and 1,150 mRNAs, respectively, corresponding to almost 20% of the yeast genome and roughly 36% of all transcriptional events each. The binding level of Yra1 targets is related to their transcriptional frequency, but that of Mex67 targets is not. Yra1-bound transcripts are enriched in mRNAs that are regulated by a number of transcription factors. Yra1- and Mex67-bound populations also show enrichment of mRNAs encoding distinct functional classes of proteins, some of which are regulated by these transcription factors. We determined that one such transcription factor, Abf1 (refs. 8-10), associates with Yra1. These results indicate a previously unidentified specificity of mRNA export factors, which coordinates the export of transcriptionally co-regulated, functional classes of transcripts, perhaps through interactions with the transcriptional machinery.