Comparative Analysis of Mesenchymal Stem Cells from Bone Marrow, Adipose Tissue, and Dental Pulp as Sources of Cell Therapy for Zone of Stasis Burns
Comparative Analysis of Mesenchymal Stem Cells from Bone Marrow, Adipose Tissue, and Dental Pulp as Sources of Cell Therapy for Zone of Stasis Burns
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DOI：
10.1080/08941939.2018.1433254
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发表时间：
2019-08-18
影响因子：
1.9
通讯作者：
Musmul, Ahmet
中科院分区：
文献类型：
--
作者：
Abbas, Ozan Luay;Ozatik, Orhan;Musmul, Ahmet
介绍：间充质干细胞（MSC）的植入已被证明对停滞区的生存有好处。然而，临床经验表明选择正确的干细胞来源和类型的重要性。因此，我们计划目前的研究进行定量比较从三个不同的来源分离的MSC，以提供有用的信息，在选择最佳的来源，看看是否关键机制是不同的人群之间的保守。方法：采用大鼠热创伤模型，比较骨髓、脂肪和牙髓来源的MSCs对热创伤的保护作用。烧伤后72 h采用组织化学、免疫组织化学和生物化学方法对瘀滞区进行评价。结果如下：烧伤创面的大体评价显示，计算的坏死面积的平均百分比之间的差异无统计学意义。组织病理学结果的半定量分级显示，损伤评分之间无显著差异。凋亡和坏死细胞死亡的免疫组化评估显示，凋亡和坏死细胞的平均数量之间的差异无统计学意义。髓过氧化物酶活性在脂肪组织组中显著降低。组织丙二醛的生化和免疫组化评估显示，组间差异无统计学意义。最后，发现牙髓组中的新生血管数量显著更高。结论：我们的研究结果表明，骨髓，脂肪组织和牙髓可以作为一个通用的供体MSC来源，用于预防烧伤创面的进展。
Introduction: The implantation of mesenchymal stem cells (MSCs) has been shown to exert benefits for the survival of the zone-of-stasis. However, the clinical experience indicates the importance of selecting the right source and type of stem cells. Therefore, we planned the current study to perform a quantitative comparison of MSCs isolated from three different sources to provide information useful in selection of the optimal source and to see whether critical mechanisms are conserved between different populations. Methods: The protective effects of MSCs derived from bone marrow, adipose tissue and dental pulp were compared in a rat model of thermal trauma. The stasis zones were evaluated 72 h after the burn using histochemistry, immunohistochemistry and biochemistry. Results: Gross evaluation of burn wounds revealed that the differences between the mean percentages of the calculated necrotic areas weren't statistically significant. Semi-quantitative grading of the histopathological findings revealed that there were no significant differences between damage scores. Immunohistochemical assessment of apoptotic and necrotic cell deaths revealed that the differences between the mean numbers of apoptotic and necrotic cells weren't statistically significant. Myeloperoxidase activity was found to be significantly lower in the adipose tissue group. Biochemical and immunohistochemical assessment of tissue malondialdehyde revealed that the differences between the groups weren't statistically significant. Finally, the number of neo-vessels in the dental pulp group was found to be significantly higher. Conclusion: Our findings suggest that bone marrow, adipose tissue and dental pulp may serve as a universal donor MSC source for the prevention of burn wound progression.